Identification of a novel human papillomavirus type 16 E1 gene variant with potentially reduced oncogenicity

Sabol, Ivan; Matovina, Mihaela; Milutin Gašperov, Nina; Grce, Magdalena (2008) Identification of a novel human papillomavirus type 16 E1 gene variant with potentially reduced oncogenicity. Journal of Medical Virology, 80 (12). pp. 2134-2140. ISSN 0146-6615

Preview

PDF - Accepted Version
Download (778kB) | Preview

Official URL: http://onlinelibrary.wiley.com/doi/10.1002/jmv.213...

Abstract

The human papillomavirus (HPV) 16 genome has been studied extensively, although no study has focused on the E1 gene that is implicated in viral DNA replication. After analyzing the E1 region of HPV 16 genomes in 429 cervical samples, 11.2% were found to contain a 63 nucleotides duplication in this region. Sequence analysis of the E6 and the E7 regions has shown that all samples containing this duplication were related to E6-G350 variant of the HPV 16 (Chi square test, P = 0.0012). A comparison of cervical lesion severity of the examinees having regular or variant E1 genes has shown that the variant group had a significantly (Fischer's exact test, P = 0.0401) lower percentage of high grade disease cases, suggesting that this particular duplication might reduce the oncogenic potential of HPV 16, and also might clarify the differences of E6-G350 variant oncogenicity observed in European populations. Albeit, a decreased incidence of high grade cervical lesions can be linked to the prevalence of multiple HPV infection, the additional decrease of those cases with the variant E1 gene versus those without (10.5% and 18.6%, respectively) can only be ascribed to the effect of this particular HPV variant. Further research is needed to clarify the biology of these HPV 16 E1 variants.

Item Type:

Article

Additional Information:

This is the peer reviewed version of the following article: Sabol, I., Matovina, M., Gasperov, N. M. and Grce, M. (2008), Identification of a novel human papillomavirus type 16 E1 gene variant with potentially reduced oncogenicity. J. Med. Virol., 80: 2134–2140, which has been published in final form at http://dx.doi.org/10.1002/jmv.21304. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Uncontrolled Keywords:

Human papillomaviruses (HPV); variant

Subjects:

BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences

Divisions:

Division of Molecular Medicine

Projects:

Project title	Project leader	Project code	Project type
Molecular profiling of metastatic breast cancer (Molekularno profiliranje metastazirajućeg tumora dojke)	Dragan Dekaris	101-0982464-2277	MZOS
Aberrant DNA methylation in HPV associated lesions (Promijenjeno stanje DNA-metilacije u HPV-povezanim oštećenjima)	Magdalena Grce	098-0982464-2510	MZOS

Depositing User:

Ivan Sabol

Date Deposited:

13 Nov 2015 17:20

URI:

http://fulir.irb.hr/id/eprint/2255

DOI:

10.1002/jmv.21304