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Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells

Klingner, Christoph; Cherian, Anoop V.; Fels, Johannes; Diesinger, Philipp M.; Aufschnaiter, Roland; Maghelli, Nicola; Keil, Thomas; Beck, Gisela; Tolić-Nørrelykke, Iva M.; Bathe, Mark; Wedlich-­Soldner, Roland (2014) Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells. Journal of Cell Biology, 207 (1). pp. 107-121. ISSN 0021-9525

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Abstract

Although cortical actin plays an important role in cellular mechanics and morphogenesis, there is surprisingly little information on cortex organization at the apical surface of cells. In this paper, we characterize organization and dynamics of microvilli (MV) and a previously unappreciated actomyosin network at the apical surface of Madin–Darby canine kidney cells. In contrast to short and static MV in confluent cells, the apical surfaces of nonconfluent epithelial cells (ECs) form highly dynamic protrusions, which are often oriented along the plane of the membrane. These dynamic MV exhibit complex and spatially correlated reorganization, which is dependent on myosin II activity. Surprisingly, myosin II is organized into an extensive network of filaments spanning the entire apical membrane in nonconfluent ECs. Dynamic MV, myosin filaments, and their associated actin filaments form an interconnected, prestressed network. Interestingly, this network regulates lateral mobility of apical membrane probes such as integrins or epidermal growth factor receptors, suggesting that coordinated actomyosin dynamics contributes to apical cell membrane organization.

Item Type: Article
Uncontrolled Keywords: EC, epithelial cell; EGFR, EGF receptor; EMT, epithelial–mesenchymal transition; HGF, hepatocyte growth factor; MHCA, myosin IIA heavy chain; MLC, myosin light chain; MSD, mean squared displacement; MV, microvilli; SEM, scanning EM; STICS, spatiotemporal image correlation spectroscopy
Subjects: NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions: Division of Molecular Biology
Depositing User: Patrik Risteski
Date Deposited: 22 Oct 2015 08:46
Last Modified: 22 Oct 2015 10:15
URI: http://fulir.irb.hr/id/eprint/2174
DOI: 10.1083/jcb.201402037

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