Stipić, Filip; Burić, Petra; Jakšić, Željko; Pletikapić, Galja; Dutour Sikirić, Maja; Zgrablić, Goran; Frkanec, Leo; Lyons, Daniel Mark (2015) Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin. Colloids and Surfaces B: Biointerfaces, 135 . pp. 481-489. ISSN 0927-7765
|
PDF
- Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (420kB) | Preview |
Abstract
With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore : nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37–3.97 μM okadaic acid range with a limit of detection of 0.25 μM. This work may have important implications for the development of new, cheap and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.
| Item Type: | Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Uncontrolled Keywords: | Biosensor; diagnostic; nanoparticle; lanthanide; immunoassay; point-of-care | ||||||||
| Subjects: | NATURAL SCIENCES > Chemistry | ||||||||
| Divisions: | Center for Marine Research Division for Marine and Enviromental Research Division of Organic Chemistry and Biochemistry Division of Physical Chemistry |
||||||||
| Projects: |
|
||||||||
| Depositing User: | Daniel Mark Lyons | ||||||||
| Date Deposited: | 26 Aug 2015 08:16 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/2098 | ||||||||
| DOI: | 10.1016/j.colsurfb.2015.07.077 |
Actions (login required)
 |
View Item |
Altmetric
Altmetric
