Stipić, Filip; Burić, Petra; Jakšić, Željko; Pletikapić, Galja; Dutour Sikirić, Maja; Zgrablić, Goran; Frkanec, Leo; Lyons, Daniel Mark (2015) Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin. Colloids and Surfaces B: Biointerfaces, 135 . pp. 481-489. ISSN 0927-7765
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With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore : nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37–3.97 μM okadaic acid range with a limit of detection of 0.25 μM. This work may have important implications for the development of new, cheap and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.
|Uncontrolled Keywords:||Biosensor; diagnostic; nanoparticle; lanthanide; immunoassay; point-of-care|
|Subjects:||NATURAL SCIENCES > Chemistry|
|Divisions:||Center for Marine Research
Division for Marine and Enviromental Research
Division of Organic Chemistry and Biochemistry
Division of Physical Chemistry
|Depositing User:||Daniel Mark Lyons|
|Date Deposited:||26 Aug 2015 08:16|
|Last Modified:||01 Nov 2016 00:15|
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