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Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin

Stipić, Filip; Burić, Petra; Jakšić, Željko; Pletikapić, Galja; Dutour Sikirić, Maja; Zgrablić, Goran; Frkanec, Leo; Lyons, Daniel Mark (2015) Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin. Colloids and Surfaces B: Biointerfaces, 135 . pp. 481-489. ISSN 0927-7765

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Abstract

With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore : nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37–3.97 μM okadaic acid range with a limit of detection of 0.25 μM. This work may have important implications for the development of new, cheap and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.

Item Type: Article
Uncontrolled Keywords: Biosensor; diagnostic; nanoparticle; lanthanide; immunoassay; point-of-care
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Center for Marine Research
Division for Marine and Enviromental Research
Division of Organic Chemistry and Biochemistry
Division of Physical Chemistry
Projects:
Project titleProject leaderProject codeProject type
Development of a nanotechnology-based targeted recognition system for bio-macromoleculesDaniel Mark Lyons02.05/17MZOS
Depositing User: Daniel Mark Lyons
Date Deposited: 26 Aug 2015 08:16
Last Modified: 01 Nov 2016 00:15
URI: http://fulir.irb.hr/id/eprint/2098
DOI: 10.1016/j.colsurfb.2015.07.077

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