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Application of functionalized lanthanide-based nanoparticles for the detection of okadaic acid-specific immunoglobulin G

Stipić, Filip; Pletikapić, Galja; Jakšić, Željko; Frkanec, Leo; Zgrablić, Goran; Burić, Petra; Lyons, Daniel Mark (2015) Application of functionalized lanthanide-based nanoparticles for the detection of okadaic acid-specific immunoglobulin G. The journal of physical chemistry. B, Condensed matter, materials, surfaces, interfaces & biophysical, 119 (4). pp. 1259-1264. ISSN 1520-6106

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Marine biotoxins are widespread in the environment and impact human health via contaminated shellfish, causing diarrhetic, amnesic, paralytic, or neurotoxic poisoning. In spite of this, methods for determining if poisoning has occurred are limited. We show the development of a simple and sensitive luminescence resonance energy transfer (LRET)-based concept which allows the detection of anti-okadaic acid rabbit polyclonal IgG (mouse monoclonal IgG1) using functionalized lanthanide-based nanoparticles. Upon UV excitation, the functionalized nanoparticles were shown to undergo LRET with fluorophore-labeled anti-okadaic acid antibodies which had been captured and bound by okadaic acid-decorated nanoparticles. The linear dependence of fluorescence emission intensity with antigen−antibody binding events was recorded in the nanomolar to micromolar range, while essentially no LRET signal was detected in the absence of antibody. These results may find applications in new, cheap, and robust sensors for detecting not only immune responses to biotoxins but also a wide range of biomolecules based on antigen−antibody recognition systems. Further, as the system is based on solution chemistry it may be sufficiently simple and versatile to be applied at point-of-care.

Item Type: Article
Uncontrolled Keywords: biotoxin; immunodetection; diagnostics; biosensor; point-of-care
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Center for Marine Research
Division for Marine and Enviromental Research
Division of Organic Chemistry and Biochemistry
Project titleProject leaderProject codeProject type
Development of a nanotechnology-based targeted recognition system for bio-macromoleculesDaniel Mark LyonsUIP-02.05/17HRZZ
Depositing User: Daniel Mark Lyons
Date Deposited: 25 Aug 2015 11:20
DOI: 10.1021/jp506382w

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