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Evaluation of cadmium-metallothionein stability constants based on voltammetric measurements

Erk, Marijana; Raspor, Biserka (1998) Evaluation of cadmium-metallothionein stability constants based on voltammetric measurements. Analytica chimica acta, 360 (1-3). pp. 189-194. ISSN 0003-2670

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Abstract

A voltammetric study on the binding properties of the commercially available rabbit liver metallothionein (MT) Sigma M 7641 for cadmium ions was performed. The capacity of metallothionein to complex cadmium and the stability constants of Cd-T complex (T denotes the apoprotein or thionein molecule) have been determined from the direct titration of the defined MT concentration with the standard CdCl2 solution in 0.59 M sodium chloride medium at pH 7.9 and 2.0. At pH 7.9 the formation of the Cd-T complex has been followed measuring the specific anodic signal height of the complex. Stability constants of Cd-T have been evaluated from the experimental data using three different procedures. The calculated apparent stability constants K=(7.6ą0.2)x108 dm3 mol-1 of Cd-T complex in 0.59 M NaCl at pH 7.9 and 25oC indicate that under the given experimental conditions all three procedures are equally applicable.

Item Type: Article
Uncontrolled Keywords: metallothionein; cadmium; apparent concentration stability constant; complexing capacity
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division for Marine and Enviromental Research
Depositing User: Marijana Erk
Date Deposited: 10 Jul 2015 11:35
Last Modified: 10 Jul 2015 11:35
URI: http://fulir.irb.hr/id/eprint/2062
DOI: 10.1016/S0003-2670(97)00729-0

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