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Cep63 and Cep152 Cooperate to Ensure Centriole Duplication

Brown, Nicola J; Marjanović, Marko; Jens, Lüders; Travis H, Stracker; Vicenzo, Constanzo (2013) Cep63 and Cep152 Cooperate to Ensure Centriole Duplication. PLoS ONE, 8 (7). e69986/1-e69986/12. ISSN 1932-6203

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Abstract

Centrosomes consist of two centrioles embedded in pericentriolar material and function as the main microtubule organising centres in dividing animal cells. They ensure proper formation and orientation of the mitotic spindle and are therefore essential for the maintenance of genome stability. Centrosome function is crucial during embryonic development, highlighted by the discovery of mutations in genes encoding centrosome or spindle pole proteins that cause autosomal recessive primary microcephaly, including Cep63 and Cep152. In this study we show that Cep63 functions to ensure that centriole duplication occurs reliably in dividing mammalian cells. We show that the interaction between Cep63 and Cep152 can occur independently of centrosome localisation and that the two proteins are dependent on one another for centrosomal localisation. Further, both mouse and human Cep63 and Cep152 cooperate to ensure efficient centriole duplication by promoting the accumulation of essential centriole duplication factors upstream of SAS-6 recruitment and procentriole formation. These observations describe the requirement for Cep63 in maintaining centriole number in dividing mammalian cells and further establish the order of events in centriole formation.

Item Type: Article
Uncontrolled Keywords: Centrosome; cell cycle; microcephaly; mitosis
Subjects: NATURAL SCIENCES > Biology
BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
Divisions: Division of Molecular Medicine
Depositing User: Marko Marjanović
Date Deposited: 08 Jul 2015 11:17
Last Modified: 08 Jul 2015 11:17
URI: http://fulir.irb.hr/id/eprint/2027
DOI: 10.1371/journal.pone.0069986

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