hrvatski jezikClear Cookie - decide language by browser settings

Human Papillomavirus in the Lesions of the Oral Mucosa According to Topography

Mravak-Stipetić, Marinka; Sabol, Ivan; Kranjčić, Josip; Knežević, Marjana; Grce, Magdalena (2013) Human Papillomavirus in the Lesions of the Oral Mucosa According to Topography. PLoS ONE, 8 (7). e69736-1-e69736-8. ISSN 1932-6203

PDF - Published Version
Available under License Creative Commons Attribution.

Download (336kB) | Preview


Background. The association between human papillomavirus (HPV) types and oral lesions has been shown in many studies. In light of the possibility of early detection of HPV genotypes in oral epithelium, and considering the significance which HPV has in the development of malignant and potentially malignant disorders of oral mucosa, the purpose of this study was to investigate the prevalence of HPV DNA in different oral lesions in the Croatian population. In addition, we wanted to elucidate whether HPV infection is associated predominantly with either the lesion or particular anatomic site of the oral cavity. Methodology/Principal Findings. The study included 246 subjects with different oral lesions, and 73 subjects with apparently healthy oral mucosa (controls). Oral lesions were classified according to their surface morphology and clinical diagnosis. Epithelial cells were collected with the cytobrush from different topographic sites in the oral cavity of oral lesions and controls. The presence of HPV DNA was evaluated by consensus and type-specific primer-directed polymerase chain reaction. The HPV positivity was detected in 17.7% of oral lesions, significantly more than in apparently healthy mucosa (6.8%), with a higher presence in benign proliferative mucosal lesions (18.6%). High-risk HPV types were predominantly found in potentially malignant oral disorders (HPV16 in 4.3% and HPV31 in 3.4%), while benign proliferative lesions as well as healthy oral mucosa contained mainly undetermined HPV type (13.6 and 6.8%, respectively). Conclusions/Significance. Distribution of positive HPV findings on oral mucosa seems to be more associated to particular anatomical site than diagnosis itself. Samples taken from vermilion border, labial commissures, and hard palate were most often HPV positive. Thus, topography plays a role in HPV prevalence findings in oral lesions. Because of the higher prevalence of the high-risk HPV types in potentially malignant oral disorders, these lesions need to be continuously controlled and treated.

Item Type: Article
Uncontrolled Keywords: Human papilloma virus - HPV; polymerase chain reaction - PCR; oral mucosa; oral lesion; potentially malignant oral disorders; topography
Subjects: BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
Divisions: Division of Molecular Medicine
Project titleProject leaderProject codeProject type
Molekularni mehanizmi nastanka prekanceroznih i kanceroznih lezija usne šupljine[122020] Marinka Mravak-Stipetić065-0982464-2532MZOS
Promijenjeno stanje DNA-metilacije u HPV-povezanim oštećenjima[138731] Magdalena Grce098-0982464-2510MZOS
Depositing User: Magdalena Grce
Date Deposited: 20 May 2015 17:17
DOI: 10.1371/journal.pone.0069736

Actions (login required)

View Item View Item


Downloads per month over past year

Increase Font
Decrease Font
Dyslexic Font