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Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue

Dragun, Zrinka; Erk, Marijana; Ivanković, Dušica; Žaja, Roko; Filipović Marijić, Vlatka; Raspor, Biserka (2010) Assessment of low-level metal contamination using the Mediterranean mussel gills as the indicator tissue. Environmental Science and Pollution Research, 17 (4). pp. 977-986. ISSN 0944-1344

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Purpose: The aim of this study was to compare the level of metal contamination in two bays in the middle part of the Eastern Adriatic coastal zone in Croatia using the gills of mussels Mytilus galloprovincialis as indicator tissue. Despite the existing sources of contamination, previous studies with caged mussels only indicated moderate metal contamination of the Kaštela Bay, contrary to the Trogir Bay in which marina and shipyard present a probable source of Cu- and Zn-contamination. Methods: The measurements of metallothioneins (MTs) and metals that induce MT synthesis (Cu, Zn and Cd) were performed in the heat-treated gill cytosol, and total proteins (TPs) in the untreated gill cytosol. MTs were determined by differential pulse voltammetry, Cu and Zn by flame atomic absorption spectrometry (AAS), Cd by graphite furnace AAS and TPs by Bradford spectrophotometric procedure. Results: The results collected in four sampling campaigns (autumn periods from 2001-2004) indicated that MT levels in mussel gills (expressed on dry mass basis 2.30.3 mg g-1) were comparable with basal levels reported in the literature (2.50.8 mg g-1). Observed interindividual, temporal and spatial MT variability could be associated with different confounding factors, such as the time of sampling, total protein concentration and mussel size rather than cytosolic levels of Cu and Zn. Metal levels, expressed on wet mass basis, in the heat-treated gill cytosol ranged from 1.33-11.31 g g-1 for Zn, from 0.72-2.96 g g-1 for Cu and from 0.036-0.100 g g-1 for Cd. The highest Zn level was measured at Vranjic (Kaštela Bay) - the site influenced by untreated domestic wastewater, while somewhat increased Zn and the highest Cu levels were found at marina and shipyard locations (Trogir Bay). The highest Cd level was measured at Inavinil (Kaštela Bay). Conclusions: The observed association of gill MT levels with several biotic and abiotic factors, limits its use as the biomarker of low-level metal exposure. Therefore, the use of the metal concentrations in the heat-treated gill cytosol of Mediterranean mussels should be considered for the assessment of the low-level metal contamination of coastal marine areas.

Item Type: Article
Uncontrolled Keywords: Mytilus galloprovincialis, gill cytosol, metallothioneins, copper, zinc, cadmium
Subjects: NATURAL SCIENCES > Biology
NATURAL SCIENCES > Biology > Ecology
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Marine Science
Divisions: Division for Marine and Enviromental Research
Project titleProject leaderProject codeProject type
CroWat project (an integrated environmental monitoring system for Croatian freshwater, estuarine, and coastal marine areas)Biserka RasporUNSPECIFIEDNorwegian Institute for Water Research (NIVA)
Stanične promjene u vodnih organizama pobuđene metalimaMarijana Erk098-0982934-2721MZOS
Ekotoksikološko značenje ABC transportnih proteina u vodenih organizama[228786] Tvrtko Smital098-0982934-2745MZOS
Depositing User: Zrinka Dragun
Date Deposited: 18 May 2015 13:04
DOI: 10.1007/s11356-009-0270-x

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