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Multielement analysis in the fish hepatic cytosol as a screening tool in the monitoring of natural waters

Dragun, Zrinka; Fiket, Željka; Vuković, Marijana; Raspor, Biserka (2013) Multielement analysis in the fish hepatic cytosol as a screening tool in the monitoring of natural waters. Environmental Monitoring and Assessment, 185 (3). pp. 2603-2614. ISSN 0167-6369

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Abstract

The possibility of direct measurement of trace elements in hepatic cytosol of European chub (Squalius cephalus) by HR ICP-MS after cytosol dilution with Milli-Q water and subsequent acidification was investigated. Due to low detection limits of this procedure, determination of 13 elements (As, Cd, Co, Cu, Fe, Mn, Mo, Pb, Sb, Sn, Sr, V, Zn) was possible in the chub hepatic cytosol, exhibiting excellent measurement repeatability in duplicates. Some of these elements were also measured by HR ICP-MS in acid digested cytosols (Cd, Co, Cu, Fe, Mn, Mo, Sr, V, Zn). Good agreement of the results obtained after sample dilution and sample digestion indicated that complex organic matrix of hepatic cytosol did not affect measurement reliability. Cytosolic concentrations of 13 trace elements in the chub liver were quantified in the following order: Fe, Zn>Cu>Mn>Mo>Sr, V, Cd>Co>As, Pb>Sn>Sb. Unlike Cd, Cu, Fe, Mn and Zn for which the cytosolic concentrations were previously reported after measurement by AAS, cytosolic concentrations of eight additional trace elements characteristic for the liver of chubs inhabiting the low contaminated river water were reported here for the first time (ng g-1): Mo 136.8-183.6, Sr 32.7-63.0, V 17.5-69.0, Co 24.3-30.7, As 9.9-29.5, Pb 5.8-35.6, Sn 5.5-12.4 and Sb 0.9-2.6. The simultaneous measurement of large number of trace elements in the cytosolic fractions of fish tissues, which comprise potentially metal-sensitive sub-cellular pools, could be beneficial as a screening tool in the monitoring of natural waters, because it would enable timely recognition of increased fish exposure to metals.

Item Type: Article
Uncontrolled Keywords: HR ICP-MS ; cytosol ; European chub ; liver ; trace elements
Subjects: NATURAL SCIENCES > Geology
NATURAL SCIENCES > Chemistry > Analytic Chemistry
NATURAL SCIENCES > Chemistry > Inorganic Chemistry
NATURAL SCIENCES > Biology
Divisions: Division for Marine and Enviromental Research
Projects:
Project titleProject leaderProject codeProject type
SAva RIver Basin : Sustainable Use, Managment and Protection of Resources - SARIBBiserka Raspor509160FP6
Stanične promjene u vodnih organizama pobuđene metalimaMarijana Erk098-0982934-2721MZOS
Biogeokemija metala u sedimentacijskim sustavima i tlima Hrvatske[85574] Goran Kniewald098-0982934-2715MZOS
Depositing User: Zrinka Dragun
Date Deposited: 11 May 2015 14:18
Last Modified: 11 May 2015 14:18
URI: http://fulir.irb.hr/id/eprint/1875
DOI: 10.1007/s10661-012-2734-6

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