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Distribution of Co, Cu, Fe, Mn, Se, Zn and Cd among cytosolic proteins of different molecular masses in gills of European chub (Squalius cephalus L.)

Krasnići, Nesrete; Dragun, Zrinka; Erk, Marijana; Raspor, Biserka (2014) Distribution of Co, Cu, Fe, Mn, Se, Zn and Cd among cytosolic proteins of different molecular masses in gills of European chub (Squalius cephalus L.). Environmental Science and Pollution Research, 21 (23). pp. 13512-13521. ISSN 0944-1344

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Abstract

The distribution of essential elements Co, Cu, Fe, Mn, Se, and Zn, and nonessential element Cd among cytosolic proteins of different molecular masses in the gills of European chub (Squalius cephalus) sampled in the moderately contaminated Sutla River in September of 2009, was studied after the protein separation by size exclusion high performance liquid chromatography (SEC-HPLC), and the metal determination in the obtained fractions by high resolution inductively coupled plasma mass spectrometry (HR ICP-MS). The aims of the study were to characterize the distribution profiles of metals within different protein categories in gills in the conditions of low metal exposure in the river water, and to compare them with the previously published hepatic profiles. The distribution profiles of analyzed metals were mainly characterized with several peaks. However, some observations could be emphasized: both Cu and Cd were eluted near metallothionein elution time ; elution time of one of Co peaks could be associated with Co-containing compound cobalamin ; increasing cytosolic Fe concentrations resulted in possible Fe binding to storage protein ferritin ; both Mn and Zn had poorly resolved peaks covering wide ranges of molecular masses and indicating their binding to various proteins ; both Zn and Se increased in protein fractions of molecular masses <5 kDa following their concentration increase in the gill cytosol ; expected clear metallothionein peak was not observed for Zn. Comparison of gill profiles with previously published hepatic profiles revealed similar and in case of some elements (e.g. Co, Fe, Mn and Se) almost identical distributions in both organs regarding elution times. Contrary, heights of obtained peaks were different, indicating possible metal binding to the same proteins in the gills and liver, but in different proportions. The results obtained in this study can be used as a basis for comparison in monitoring studies, for identification of changes that would occur after exposure of chub to increased metal concentrations.

Item Type: Article
Additional Information: The final publication is available at Springer via http://dx.doi.org/10.1007/s11356-014-3274-0
Uncontrolled Keywords: cytosolic proteins; European chub; gills; HR ICP-MS; metals; SEC-HPLC
Subjects: NATURAL SCIENCES > Chemistry
NATURAL SCIENCES > Chemistry > Analytic Chemistry
NATURAL SCIENCES > Biology
NATURAL SCIENCES > Interdisciplinary Natural Sciences > Environmental Science
Divisions: Division for Marine and Enviromental Research
Projects:
Project titleProject leaderProject codeProject type
Stanične promjene u vodnih organizama pobuđene metalimaMarijana Erk098-0982934-2721MZOS
Depositing User: Zrinka Dragun
Date Deposited: 18 May 2015 09:12
Last Modified: 25 Jan 2016 12:26
URI: http://fulir.irb.hr/id/eprint/1873
DOI: 10.1007/s11356-014-3274-0

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