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Endogenous auxin profile in the Christmas rose (Helleborus niger L.) flower and fruit: Free and amide conjugated IAA

Brcko, Ana; Penčik, Aleš; Magnus, Volker; Prebeg, Tatjana; Mlinarić, Selma; Antunović, Jasenka; Lepeduš, Hrvoje; Cesar, Vera; Strnad, Miroslav; Rolčik, Jakub; Salopek Sondi, Branka (2012) Endogenous auxin profile in the Christmas rose (Helleborus niger L.) flower and fruit: Free and amide conjugated IAA. Journal of Plant Growth Regulation, 31 (1). pp. 63-78. ISSN 0721-7595

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Abstract

The reproductive development of the Christmas rose (Helleborus niger L.) is characterized by an uncommon feature in the world of flowering plants: after fertilization the white perianth becomes green, photosynthetically active and persists during fruit development. In the flowers in which fertilization was prevented by emasculation (unfertilized) or entire reproductive organs were removed (depistillated), the elongation of the peduncle was reduced by 20 to 30%, and vascular development, particularly lignin deposition in sclerenchyma was arrested. Chlorophyll accumulation in sepals and their photosynthetic efficacy was up to 80% lower in comparison to fertilized flowers. Endogenous auxins were investigated in floral and fruit tissues and their potential roles in these processes were discussed. Analytical data of free indole-3-acetic acid (IAA), indole-3-ethanol (IEt), and seven amino acid conjugates were afforded by LC-MS/MS in floral tissues of fertilized as well as unfertilized and depistillated flowers. Among amino acid conjugates novel ones with Val, Gly, and Phe, were identified and quantified in the anthers, and in the fruit during development. Reproductive organs before fertilization, followed by developing fruit at post-anthesis were the main source of auxin. Tissues of unfertilized and depistillated flowers accumulated significantly lower level of auxin. Upon depistillation, auxin content in the peduncle and sepal was decreased to 4% and 45%, respectively, in comparison to fruit-bearing flowers. This study suggests that auxin arising in developing fruit may participate, in part, in coordination of the Christmas rose peduncle elongation and its vascular development.

Item Type: Article
Uncontrolled Keywords: auxin; iIndole-3-acetic acid; amide conjugates; Christmas rose; Helleborus niger L.; flower and fruit development; Perianth greening, Peduncle elongation, vascular system
Subjects: NATURAL SCIENCES
NATURAL SCIENCES > Biology
NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology
Divisions: Division of Molecular Biology
Projects:
Project titleProject leaderProject codeProject type
Molekularna regulacija biljnog razvitkaBranka Salopek-Sondi098-0982913-2829MZOS
Utjecaj oksidativnog stresa na organizaciju i funkciju biljnih stanica i tkivaHrvoje Lepeduš073-0731674-1673MZOS
Fiziologija stresa i gospodarska svojstva različitih kultivara pšenice i ječmaJosip Kovačević073-0731674-0552MZOS
Stanična i tkivna diferencijacija tijekom razvoja biljnih organaVera Cesar073-0731674-0841MZOS
Regulatorni mehanizmi fotosinteze i diferencijacija plastidaHrvoje Fulgosi098-0982913-2838MZOS
Depositing User: Branka Salopek Sondi
Date Deposited: 20 Dec 2016 09:17
Last Modified: 20 Dec 2016 09:17
URI: http://fulir.irb.hr/id/eprint/1850
DOI: 10.1007/s00344-011-9220-1

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