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Uniform distribution of satellite DNA variants on the chromosomes of tenebrionid species Alphitobius diaperinus and Tenebrio molitor

Bruvo Mađarić, Branka; Plohl, Miroslav; Ugarković, Đurđica (1995) Uniform distribution of satellite DNA variants on the chromosomes of tenebrionid species Alphitobius diaperinus and Tenebrio molitor. Hereditas, 123 (1). pp. 69-75. ISSN 0018-0661

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The chromosomes of tenebrionid species Alphitobius diaperinus contain large blocks of pericentromerically located constitutive heterochromatin, as revealed by C-banding procedure. As previously reported, satellite DNA of this species is composed of two related monomeric units organized in three satellite subfamilies. In order to analyze the chromosomal location of the satellite DNA and the distribution of monomeric variants within it, and compare it with the distribution of monomer variants in Tenebrio molitor satellite DNA, the methods of in situ hybridization and restriction enzyme/nick translation were performed. Fluorescent in situ hybridization with the entire satellite DNA reveals the pericentromerically located signals on all chromosomes of the complement, coinciding with heterochromatic blocks. Results of fluorescent in situ hybridization with particular monomeric variants and of in situ restriction enzyme/nick translation show that monomeric variants are homogeneously dispersed within the entire satellite DNA. The spreading of satellite monomeric variants of the related species T. molitor within the pericentromeric heterochromatin of the entire complement, is demonstrated using the method of in situ restriction enzyme/nick translation. Although the complexity of organization of satellite DNAs is quite distinct in these two species, obtained results suggest similar efficiency of mechanisms of spreading and homogenization resulting in random chromosomal distribution of their satellite variants.

Item Type: Article
Uncontrolled Keywords: tandemly repeated DNA; centromeric heterochromatin; In situ hybridization; nick translation; sequence; beetle; digestion
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Molecular Biology
Depositing User: Đurđica Ugarković
Date Deposited: 02 Mar 2015 15:39
Last Modified: 07 May 2015 12:44
DOI: 10.1111/j.1601-5223.1995.00069.x

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