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The effects of zolpidem treatment on GABAA receptors in cultured cerebellar granule cells: Changes in functional coupling

Vlainić, Josipa; Švob Štrac, Dubravka; Jazvinšćak Jembrek, Maja; Vlainić, Toni; Peričić, Danka (2012) The effects of zolpidem treatment on GABAA receptors in cultured cerebellar granule cells: Changes in functional coupling. Life Sciences, 90 (23/24). pp. 889-894. ISSN 0024-3205

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Abstract

Hypnotic zolpidem is a positive allosteric modulator of γ-aminobutyric acid (GABA) action, with preferential although not exclusive binding for α1 subunit-containing GABAA receptors. The pharmacological profile of this drug is different from that of classical benzodiazepines, although it acts through benzodiazepine binding sites at GABAA receptors. The aim of this study was to further explore the molecular mechanisms of GABAA receptor induction by zolpidem. In the present study, we explored the effects of two-day zolpidem (10 µM) treatment on GABAA receptors on the membranes of rat cerebellar granule cells (CGCs) using [3H]flunitrazepam binding and semiquantitative PCR analysis. Two-day zolpidem treatment of CGCs did not significantly affect the maximum number (Bmax) of [3H]flunitrazepam binding sites or the expression of α1 subunit mRNA. However, as shown by decreased GABA [3H]flunitrazepam binding, two-day exposure of CGCs to zolpidem caused functional uncoupling of GABA and benzodiazepine binding sites at GABAA receptor complexes. Significance: If functional uncoupling of GABA and benzodiazepine binding sites at GABAA receptors is the mechanism responsible for the development of tolerance following long-term administration of classical benzodiazepines, chronic zolpidem treatment may induce tolerance.

Item Type: Article
Uncontrolled Keywords: zolpidem; cerebellar granule cells; GABAA receptor; [3H]flunitrazepam binding; gene expression; tolerance
Subjects: BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences > Pharmacology
BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences > Neuroscience
Divisions: Division of Molecular Medicine
Projects:
Project titleProject leaderProject codeProject type
Stres, GABA-A receptori i mehanizmi djelovanja neuropsihofarmaka[258591] Dubravka Švob Štrac098-0000000-2448MZOS
Depositing User: Josipa Vlainić
Date Deposited: 30 Oct 2014 13:20
Last Modified: 30 Oct 2014 13:20
URI: http://fulir.irb.hr/id/eprint/1565
DOI: 10.1016/j.lfs.2012.04.021

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