Vlainić, Josipa; Švob Štrac, Dubravka; Jazvinšćak Jembrek, Maja; Vlainić, Toni; Peričić, Danka (2012) The effects of zolpidem treatment on GABAA receptors in cultured cerebellar granule cells: Changes in functional coupling. Life Sciences, 90 (23/24). pp. 889-894. ISSN 0024-3205
Abstract
Hypnotic zolpidem is a positive allosteric modulator of γ-aminobutyric acid (GABA) action, with preferential although not exclusive binding for α1 subunit-containing GABAA receptors. The pharmacological profile of this drug is different from that of classical benzodiazepines, although it acts through benzodiazepine binding sites at GABAA receptors. The aim of this study was to further explore the molecular mechanisms of GABAA receptor induction by zolpidem. In the present study, we explored the effects of two-day zolpidem (10 µM) treatment on GABAA receptors on the membranes of rat cerebellar granule cells (CGCs) using [3H]flunitrazepam binding and semiquantitative PCR analysis. Two-day zolpidem treatment of CGCs did not significantly affect the maximum number (Bmax) of [3H]flunitrazepam binding sites or the expression of α1 subunit mRNA. However, as shown by decreased GABA [3H]flunitrazepam binding, two-day exposure of CGCs to zolpidem caused functional uncoupling of GABA and benzodiazepine binding sites at GABAA receptor complexes. Significance: If functional uncoupling of GABA and benzodiazepine binding sites at GABAA receptors is the mechanism responsible for the development of tolerance following long-term administration of classical benzodiazepines, chronic zolpidem treatment may induce tolerance.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | zolpidem; cerebellar granule cells; GABAA receptor; [3H]flunitrazepam binding; gene expression; tolerance | ||||||||
| Subjects: | BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences > Pharmacology BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences > Neuroscience |
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| Divisions: | Division of Molecular Medicine | ||||||||
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| Depositing User: | Josipa Vlainić | ||||||||
| Date Deposited: | 30 Oct 2014 13:20 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/1565 | ||||||||
| DOI: | 10.1016/j.lfs.2012.04.021 |
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