Jurković, Marta; Ferger, Matthias; Drašković, Isabela; Marder, Todd B.; Piantanida, Ivo (2023) Triarylborane-“Click” Fluorescent Tag for Orthogonal Amino Acid Labelling, Interactions with DNA, Protein, and Cyclodextrins. Pharmaceuticals, 16 (9). ISSN 1424-8247
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Abstract
The innovative design of a triarylborane (TB)-dye with one NMe2-alkylated (propargylated) group and one NMe2 group yielded a system that is both an NMe2 π-donor and an inductive NMe2-alkyl cationic acceptor. Consequently, the new TB-dye was highly sensitive to a “click” reaction with an azide-substituted lysine side chain (yielding TB-lysine), resulting in a bathochromic shift of emission of 100 nm. In addition, fluorene attached to the lysine C-terminus showed FRET with the TB-chromophore, also sensitive to interactions with targets. Both the TB-dye and TB-lysine showed high affinities towards both DNA and proteins, reporting binding by an opposite fluorimetric response for DNA/RNA (quenching) vs. BSA (increase). Thus, the novel TB-dye is an ideal fluorimetric probe for orthogonal incorporation into bio-targets by “click” reactions due to fluorescence reporting of the progress of the “click” reaction and further sensing of the binding site composition. The TB-dye is moderately toxic to human cell lines after 2–3 days of exposure, but efficiently enters cells in 90 min, being non-toxic at short exposure. The most important product of the “click” reaction, TB-lysine, was non-toxic to cells and showed equal distribution between mitochondria and lysosomes. Further studies would focus particularly on the very convenient monitoring of the progress of “click” conjugation of the TB-dye with biorelevant targets inside living cells by confocal microscopy.
| Item Type: | Article | ||||||||||||
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| Uncontrolled Keywords: | triarylborane; “click”; DNA sensing; protein sensing; fluorescence; cyclodextrin binding | ||||||||||||
| Subjects: | NATURAL SCIENCES > Chemistry > Analytic Chemistry NATURAL SCIENCES > Chemistry > Organic Chemistry NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology |
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| Divisions: | Division of Molecular Biology Division of Organic Chemistry and Biochemistry |
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| Depositing User: | Ivana Vuglec | ||||||||||||
| Date Deposited: | 16 Apr 2026 08:59 | ||||||||||||
| URI: | http://fulir.irb.hr/id/eprint/11728 | ||||||||||||
| DOI: | 10.3390/ph16091208 |
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