hrvatski jezikClear Cookie - decide language by browser settings

Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture

Peharec Štefanić, Petra; Smolko, Ana; Horvatić, Anita; Cindrić, Mario; Krsnik-Rasol, Marijana; Balen, Biljana (2013) Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture. Croatica Chemica Acta, 86 (1). pp. 27-37. ISSN 0011-1643

[img] PDF - Published Version
Download (3MB)

Abstract

In this study cellular proteins of in vitro grown horseradish (Armoracia lapathifolia Gilib.) tissues of different morphologies, leaves (L), teratoma (TM) and tumor (TN), were separated by one-dimensional (1-DE) and two-dimensional polyacrylamide gel electrophoresis (2-DE) in order to reveal developmentally specific proteins. Moreover, proteins were subjected to analyses by lectin assays to reveal whether the morphological differences were reflected in protein glycosylation. 1-DE glycoprotein pattern showed substantial distinctions between horseradish tissues of different morphologies, which was in contrast to silver stained protein profiles, where only slightly qualitative changes were observed. 2-DE revealed more differences in expression of cellular proteins between examined tissues in comparison to 1-DE, while 2-DE blots confirmed quantitative difference in expression of 43 kDa glycoprotein between investigated tissues. Mass spectrometry analysis identified this protein as a typical horseradish peroxidase. Obtained results imply that the oligosaccharide structures could be important determinants of specificities of horseradish tissue morphology.

Item Type: Article
Uncontrolled Keywords: morphogenesis; horseradish; glycoproteins; lectine assay; tissue culture; 2-DE; arabinogalactan-proteins; developmental-stage; embryo development; mass-spectrometry; in-vitro; glycosylation; tobacco; markers; binding; lectin
Subjects: NATURAL SCIENCES > Chemistry
Divisions: Division of Molecular Biology
Division of Molecular Medicine
Projects:
Project titleProject leaderProject codeProject type
Diferencijalna ekspresija proteina u biljnim stanicama[222686] Biljana Balen119-1191196-1200MZOS
Depositing User: Mario Cindrić
Date Deposited: 28 Nov 2013 14:16
Last Modified: 07 Mar 2014 09:55
URI: http://fulir.irb.hr/id/eprint/1172
DOI: 10.5562/cca2078

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year