Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture

Peharec Štefanić, Petra; Smolko, Ana; Horvatić, Anita; Cindrić, Mario; Krsnik-Rasol, Marijana; Balen, Biljana (2013) Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture. Croatica Chemica Acta, 86 (1). pp. 27-37. ISSN 0011-1643

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Abstract

In this study cellular proteins of in vitro grown horseradish (Armoracia lapathifolia Gilib.) tissues of different morphologies, leaves (L), teratoma (TM) and tumor (TN), were separated by one-dimensional (1-DE) and two-dimensional polyacrylamide gel electrophoresis (2-DE) in order to reveal developmentally specific proteins. Moreover, proteins were subjected to analyses by lectin assays to reveal whether the morphological differences were reflected in protein glycosylation. 1-DE glycoprotein pattern showed substantial distinctions between horseradish tissues of different morphologies, which was in contrast to silver stained protein profiles, where only slightly qualitative changes were observed. 2-DE revealed more differences in expression of cellular proteins between examined tissues in comparison to 1-DE, while 2-DE blots confirmed quantitative difference in expression of 43 kDa glycoprotein between investigated tissues. Mass spectrometry analysis identified this protein as a typical horseradish peroxidase. Obtained results imply that the oligosaccharide structures could be important determinants of specificities of horseradish tissue morphology.

Item Type:

Article

Uncontrolled Keywords:

morphogenesis; horseradish; glycoproteins; lectine assay; tissue culture; 2-DE; arabinogalactan-proteins; developmental-stage; embryo development; mass-spectrometry; in-vitro; glycosylation; tobacco; markers; binding; lectin

Subjects:

NATURAL SCIENCES > Chemistry

Divisions:

Division of Molecular Biology
Division of Molecular Medicine

Projects: