Histochemical Study of Enzyme Activity in the Digestive Tract of the Small-Spotted Catshark (Scyliorhinus canicula) and the Smooth-Hound (Mustelus mustelus)

Devčić, Lucija; Vlahek, Ivan; Palić, Magdalena; Benko, Valerija; Faraguna, Siniša; Lovrić, Marin; Valić, Damir; Kužir, Snježana (2025) Histochemical Study of Enzyme Activity in the Digestive Tract of the Small-Spotted Catshark (Scyliorhinus canicula) and the Smooth-Hound (Mustelus mustelus). Fishes, 10 (8). ISSN 2410-3888

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Abstract

The small-spotted catshark and the smooth-hound are cartilaginous, carnivorous fish with similar depth ranges in their habitats. These two species are among the most abundant elasmobranchs in the Adriatic Sea and are frequently caught by local fishermen using longline fishing. Despite their ecological similarities, little is known about the physiological differences in their digestive processes. The study of enzymatic digestion in these ecologically relevant species helps to fill the knowledge gap in the understanding of nutrient processing in cartilaginous fish. Therefore, the aim of this study was to determine, measure and compare the enzymatic activity of alkaline phosphatase, acid phosphatase, non-specific esterase and aminopeptidase. Fish were caught in the central part of the Adriatic Sea between 2021 and 2023. A total of 60 adult individuals were analyzed, with samples taken from six parts of the digestive tract. Histochemical analysis of 1440 slides revealed clear differences in enzyme activity between the two species. In the small-spotted catshark, cellular protein degradation was most pronounced in esophagus, posterior stomach and rectum, whereas in the smooth-hound, it was concentrated in posterior stomach and spiral intestine. Cellular digestion of lipids in the small-spotted catshark appears to occur primarily in the stomach. The results of this study provide new insights into the distribution of cellular digestive enzymes in cartilaginous fish and emphasize the importance of studying the entire digestive tract as an integrated system rather than focusing on individual parts. This study fills an important knowledge gap and contributes to a deeper understanding of digestive physiology, which in turn has implications for species conservation and biological variability.

Item Type:	Article
Uncontrolled Keywords:	small-spotted catshark; smooth-hound; cartilaginous fish; histochemistry; cellular digestion
Subjects:	NATURAL SCIENCES
Divisions:	Division for Marine and Enviromental Research
Depositing User:	Ana Zečević
Date Deposited:	14 Apr 2026 12:49
URI:	http://fulir.irb.hr/id/eprint/11704
DOI:	10.3390/fishes10080386

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