cfDNA Methylation in Liquid Biopsies as Potential Testicular Seminoma Biomarker

Raos, Dora; Oršolić, Davor; Mašić, Silvija; Tomić, Miroslav; Krasić, Jure; Tomašković, Igor; Gabaj, Nora Nikolac; Gelo, Nina; Kaštelan, Željko; Kuliš, Tomislav; Bojanac, Ana Katušić; Barešić, Anja; Ulamec, Monika; Ježek, Davor; Sinčić, Nino (2022) cfDNA Methylation in Liquid Biopsies as Potential Testicular Seminoma Biomarker. Epigenomics, 14 (23). pp. 1493-1507. ISSN 1750-1911

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Abstract

Plain language summarySeminoma belongs to testicular cancer, which represents a common malignancy among men of reproductive age. Diagnosis of seminoma is a multistep process that also includes checking tumor biomarkers from blood. However, these biomarkers are not specific for seminoma and to conclude a definite diagnosis of seminoma immunohistochemical analysis is needed, which requires the removal of a whole or partial testicle. Therefore, there is a need for novel, noninvasive biomarkers. cfDNA is the most extensively investigated source of minimally invasive tumor markers. Therefore, this study investigated cfDNA methylation of six genes as potential noninvasive biomarkers for the management of seminoma patients. By examining CpG sites of selected genes by pyrosequencing, the authors detected significant differences. However, receiver operating characteristic curve analysis showed modest results. Therefore, the authors tested possible panels of significantly different CpGs and detected two possible DNA methylation panels for seminoma screening. These findings suggest the further investigation of possible epigenetic biomarkers for seminoma patient management from liquid biopsies. Background: Seminoma is a testicular tumor type, routinely diagnosed after orchidectomy. As cfDNA represents a source of minimally invasive seminoma patient management, this study aimed to investigate whether cfDNA methylation of six genes from liquid biopsies, have potential as novel seminoma biomarkers. Materials & methods: cfDNA methylation from liquid biopsies was assessed by pyrosequencing and compared with healthy volunteers' samples. Results: Detailed analysis revealed specific CpGs as possible seminoma biomarkers, but receiver operating characteristic curve analysis showed modest diagnostic performance. In an analysis of panels of statistically significant CpGs, two DNA methylation panels emerged as potential seminoma screening panels, one in blood CpG8/CpG9/CpG10 (KITLG) and the other in seminal plasma CpG1(MAGEC2)/CpG1(OCT3/4). Conclusion: The presented data promote the development of liquid biopsy epigenetic biomarkers in the screening of seminoma patients. Tweetable abstractNovel research of potential epigenetic biomarkers for the management of seminoma patients from liquid biopsies.

Item Type:	Article
Uncontrolled Keywords:	DNA methylation ; biomarkers ; cfDNA ; liquid biopsy ; seminoma
Subjects:	NATURAL SCIENCES > Interdisciplinary Natural Sciences BIOMEDICINE AND HEALTHCARE > Basic Medical Sciences
Divisions:	Division of Electronics
Projects:	Project title Project leader Project code Project type Znanstveni centar izvrsnosti za reproduktivnu i regenerativnu medicinu – istraživanja novih platormi i potencijala-CERRM Siniša Volarević; Slobodan Vukičević; Davor Ježek KK.01.1.1.01.0008 EK Epigenetički biomarkeri u krvi i ejakulatu bolesnika sa seminomom testisa-epiSem Davor Ježek IP-2016-06-3692 HRZZ
Depositing User:	Kristina Ciglar
Date Deposited:	02 Apr 2026 13:58
URI:	http://fulir.irb.hr/id/eprint/11577
DOI:	10.2217/epi-2022-0331

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