Maleš, Petra; Brkljača, Zlatko; Portada, Tomislav; Bakarić, Danijela; Crnolatac, Ivo (2025) 9,10-Diphenylanthracene based fluorescent probes as reporters of lipid membrane phase transitions. Dyes and Pigments, 235 . ISSN 0143-7208
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Abstract
The two fluorescent probes 2-(2-bromoethoxy)-9,10-diphenylanthracene and 9,10-diphenylanthracene were evaluated for their suitability to report temperature-induced phase transitions in phospholipid formulations. Multilamellar liposomes were formed from two different lipids, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine and the probes were embedded in the bilayers. The fluorescence spectra of the embedded probes were collected over the temperature range of the lipid phase transitions. The transition temperatures were determined by differential scanning calorimetry and temperature-dependent fluorescence spectroscopy. The fluorescence lifetimes of the emitting species were calculated by time-resolved fluorescent spectroscopy in gel and fluid phases. The molecular dynamics simulations were employed to describe the impact of the probe positioning within the bilayer on their emitting properties. The probes did not significantly impact the physicochemical characteristics of the vesicles. Both probes can report on main lipid phase transitions in the two types of liposomes. However, upon transition from gel to fluid phase, the emitting species show longer-lived fluorescence.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (dppc) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (dppe); fluorescent probes; 9,10-diphenylanthracene (dpa) and 2-(2-bromoethoxy)-9,10-diphenylanthracene; differential scanning calorimetry; temperature-dependent fluorescent spectroscopy; time-resolved fluorescence; molecular dynamics simulations | ||||||||
| Subjects: | NATURAL SCIENCES NATURAL SCIENCES > Chemistry NATURAL SCIENCES > Chemistry > Physical Chemistry NATURAL SCIENCES > Chemistry > Organic Chemistry |
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| Divisions: | Division of Organic Chemistry and Biochemistry | ||||||||
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| Depositing User: | Danijela Bakarić | ||||||||
| Date Deposited: | 15 Jan 2026 11:54 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/10806 | ||||||||
| DOI: | 10.1016/j.dyepig.2024.112608 |
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