CENP-E initiates chromosome congression by opposing Aurora kinases to promote end-on attachments

Vukušić, Kruno; Tolić, Iva M. (2025) CENP-E initiates chromosome congression by opposing Aurora kinases to promote end-on attachments. Nature Communications, 16 (8537). ISSN 2041-1723

PDF - Published Version - article Available under License Creative Commons Attribution. Download (13MB)

Abstract

Accurate cell division relies on rapid chromosome congression. The kinetochore motor protein CENP-E/kinesin-7 is uniquely required for congression of polar chromosomes. It is currently assumed that CENP-E drives congression by gliding kinetochores along microtubules independently of their biorientation. Here, by studying chromosome movement under different levels of CENP-E activity, we favor an alternative model in which CENP-E initiates congression by promoting stabilization of end-on attachments. In this way, CENP-E accelerates congression initiation without significantly contributing to subsequent movement. Stabilization of end-on attachments on polar chromosomes without CENP-E is delayed due to Aurora kinase-mediated hyperphosphorylation of microtubule-binding proteins and expansion of the fibrous corona. CENP-E counters this by reducing Aurora B-mediated phosphorylation in a BubR1-dependent manner, thereby stabilizing initial end-on attachments, facilitating removal of the fibrous corona, and triggering biorientation-dependent chromosome movement. These findings support a unified model of chromosome movement in which congression is intrinsically coupled to biorientation.

Item Type:	Article
Uncontrolled Keywords:	kinetochore-microtubule attachments; checkpoint protein; mitotic check point; fibrous corona
Subjects:	NATURAL SCIENCES > Biology
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Molecular origins of aneuploidies in healthy and diseased human tissues-ANEUPLOIDY Iva Tolić; Nenad Pavin 855158 EK Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini-IPSted Iva Tolić KK.01.1.1.04.0057 EK Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri-QuantiXLie Hrvoje Buljan; Pavle Pandžić; Vit Tuček; Tomislav Marketin KK.01.1.1.01.0004 EK Kako starost centrosoma utječe na stvaranje, popravak i segregaciju polarnih kromosoma Iva Tolić 2022-10-9344 HRZZ (IPCH)
Depositing User:	Iva Marija Tolić
Date Deposited:	07 Jan 2026 13:43
URI:	http://fulir.irb.hr/id/eprint/10736
DOI:	10.1038/s41467-025-64148-w

Actions (login required)

View Item