Super-Resolution Imaging of Mitotic Spindle Microtubules Using STED Microscopy

Koprivec, Isabella; Štimac, Valentina; Tolić, Iva M. (2025) Super-Resolution Imaging of Mitotic Spindle Microtubules Using STED Microscopy. In: The Mitotic Spindle. Methods in Molecular Biology, 2872 . Springer Nature, Springer Protocols, pp. 3-18.

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Abstract

Stimulated emission depletion (STED) microscopy is a powerful super-resolution imaging technique that only recently entered the field of mitosis, where it proved to be invaluable for studying various microtubule classes, kinetochore-microtubule attachments and chromosome segregation errors. Here, we describe immunofluorescence combined with STED microscopy as a method for analyzing microtubules and kinetochore-microtubule attachments in human mitotic spindles. We also describe live-cell STED microscopy as a method for single-plane short-term imaging of transient processes in crowded spindle areas. Finally, we outline image analysis approaches for the quantitative assessment of microtubule bundles within the spindle.

Item Type:	Book Section
Uncontrolled Keywords:	STED microscopy; Cell division; Mitosis; Mitotic spindle; Microtubules; Kinetochores; Chromosomes; Segregation errors
Subjects:	NATURAL SCIENCES > Biology
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Molecular origins of aneuploidies in healthy and diseased human tissues-ANEUPLOIDY Iva Tolić; Nenad Pavin 855158 EK Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena-SpindleForm Iva Tolić; Nenad Pavin PZS-2019-02-7653 HRZZ Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini-IPSted Iva Tolić KK.01.1.1.04.0057 EK Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri-QuantiXLie Hrvoje Buljan; Pavle Pandžić; Vit Tuček; Tomislav Marketin KK.01.1.1.01.0004 EK
Depositing User:	Iva Marija Tolić
Date Deposited:	07 Jan 2026 14:20
URI:	http://fulir.irb.hr/id/eprint/10721

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