Vujović, Tamara; Babić Brčić, Sanja; Paradžik, Tina; Miloš, Marija; Galić Perečinec, Maja; Vranjković, Lucija; Starcevic, Antonio; Bojanić, Krunoslav; Čož-Rakovac, Rozelindra; Ivančić Šantek, Mirela; Strunjak-Perović, Ivančica (2025) Microalga Chlamydomonas reinhardtii cultivation and biomass processing optimization for biological activity improvement. Algal Research, 92 . ISSN 2211-9264
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Abstract
Chlamydomonas reinhardtii is a unicellular green alga with promising biotechnological potential. This study systematically examined the interdependence between post-cultivation processing and chemical composition and food-relevant bioactivity of C. reinhardtii. After cultivation optimization, we determined pigment, protein, and lipid content and assessed biological activity through various in vitro assays and in vivo zebrafish toxicity testing. Results showed strong antioxidative, antidiabetic, and antimicrobial properties, particularly when biomass was dried at temperatures below 60 °C. This indicates that important metabolites, such as fatty acids and terpenoids, are better preserved during oven-drying than lyophilization. Bioactive compounds were detected by Q-TOF and linked with observed activities. This is the first comprehensive study combining cultivation optimization, processing methods, and bioactivity evaluation of C. reinhardtii. Our findings show that C. reinhardtii could be used to develop functional food and pharmaceutical products, and that preservation of bioactive compounds is critical for the greatest efficacy and product quality.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | Microalgal biomass production; Ultrasound-assisted extraction; Fractionation; Chemical analysis; Enzyme inhibition; Zebrafish embryotoxicity | ||||||||
| Subjects: | BIOTECHNICAL SCIENCES BIOTECHNICAL SCIENCES > Interdisciplinary Technical Sciences INTERDISCIPLINARY AREAS OF KNOWLEDGE |
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| Divisions: | Division for Marine and Enviromental Research Division of Materials Chemistry Division of Physical Chemistry |
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| Depositing User: | Ivančica Strunjak-Perović | ||||||||
| Date Deposited: | 24 Nov 2025 10:49 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/10223 | ||||||||
| DOI: | 10.1016/j.algal.2025.104354 |
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