Monitoring lipid phase transition temperatures using fluorescent probes and temperature-dependent fluorescence spectroscopy

Brkljača, Zlatko; Čehić, Mirsada; Portada, Tomislav; Butumović, Marija; Bakarić, Danijela; Crnolatac, Ivo (2022) Monitoring lipid phase transition temperatures using fluorescent probes and temperature-dependent fluorescence spectroscopy. Dyes and Pigments, 206 . ISSN 0143-7208

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Sažetak

The response of fluorescent dyes incorporated in multilamellar liposomes was evaluated for their applicability in monitoring thermal-induced changes in liposomes. Five fluorescent probes, diverse in polarity, lipophilicity, and consequently their position within the lipid multibilayers constituted from 1,2-dipalmitoyl-sn-glycero-3- phosphocholine (DPPC), were used to record temperature-dependent fluorescence spectra of the liposomal suspensions. The responses of the dyes were analysed in a multivariate fashion to determine pre- and main phase transition temperatures of DPPC. Laurdan® and 1-chloro-9,10-bis(phenylethynyl)anthracene9,10-bis (phenylethynyl)anthracene register both phase transitions, while 9,10-diphenylanthracene and 9,10-bis (phenylethynyl)anthracene report only pretransition. According to the differential scanning calorimetry (DSC) measurements, all suspensions display both phase transitions that resemble DSC curve of pure DPPC. The positions of the dyes within the bilayers were determined by molecular dynamics simulations.

Vrsta građe:	Rad objavljen u časopisu
Ključne riječi:	1, 2-Dipalmitoyl-sn-glycero-3-phosphocholine ; Fluorescent dyes ; Differential scanning calorimetry ; Fluorescence spectroscopy ; Multivariate curve analysis ; Molecular dynamics simulations
Područja:	PRIRODNE ZNANOSTI > Kemija > Fizikalna kemija PRIRODNE ZNANOSTI > Kemija > Teorijska kemija PRIRODNE ZNANOSTI > Kemija > Analitička kemija PRIRODNE ZNANOSTI > Kemija > Organska kemija PRIRODNE ZNANOSTI > Kemija > Primijenjena kemija PRIRODNE ZNANOSTI > Interdisciplinarne prirodne znanosti TEHNIČKE ZNANOSTI > Kemijsko inženjerstvo
Zavodi:	Zavod za organsku kemiju i biokemiju
Projekti:	Naziv projekta Voditelj projekta Šifra projekta Vrsta projekta Model demijelinizacije na molekulskoj skali pri fiziološkim i patološkim uvjetima-DEMYMOLSCALE Danijela Bakarić UIP-2020-02-7669 HRZZ Identifikacija interaktoma paralognih proteina SSB u višestaničnom prokariotu, Streptomyces coelicolor-id_ROBUST Dušica Vujaklija IP-2018-01-1754 HRZZ
Pohranio:	Ivo Crnolatac
Datum pohrane:	06 Feb 2024 13:41
URI:	https://fulir.irb.hr/id/eprint/8495
DOI:	10.1016/j.dyepig.2022.110621

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