Šarić, Ana; Sobočanec, Sandra; Mačak Šafranko, Željka; Popović Hadžija, Marijana; Bagarić, Robert; Farkaš, Vladimir; Švarc, Alfred; Marotti, Tatjana; Balog, Tihomir (2015) Diminished resistance to hyperoxia in brains of reproductively senescent female CBA/H mice. Medical Science Monitor Basic Research, 21 . pp. 191-199. ISSN 2325-4394
|
PDF
- Obnovljena verzija
- članak
Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (419kB) | Preview |
Sažetak
Background: We have explored sex differences in ability to maintain redox balance during acute oxidative stress in mice brain. We aimed to study if there were differences in oxidative/antioxidative status upon hyperoxia in brain of reproductively senescent CBA/H mice in order to elucidate some of the possible mechanisms of lifespan regulation. Material and Methods: The brains of 12 months old male and female CBA/H mice (n=9 per sex and treatment) subjected to 18h hyperoxia were evaluated for lipid peroxidation (LPO), antioxidative enzyme expression and activity - superoxide dismutase 1 and 2 (Sod-1, Sod-2), catalase (Cat), glutathione peroxidase 1 (Gpx- 1), heme-oxygenase 1 (Ho-1), nad NF-E2-related factor 2 (Nrf2), and for 2-deoxy-2-[18F] fluoro-D-glucose (18FDG) uptake. Results: No increase in LPO was observed after hyperoxia, regardless of sex. Expression of Nrf-2 showed significant downregulation in hyperoxia-treated males (p=0.001), and upregulation in hyperoxia-treated females (p=0.023). Also, in females hyperoxia upregulated Sod-1 (p=0.046), and Ho-1 (p=0.014) genes. SOD1 protein was upregulated in both sexes after hyperoxia (p=0.009 for males and p=0.011 for females). SOD2 protein was upregulated only in females (p=0.008) while CAT (p=0.026) and HO-1 (p=0.042) proteins were increased after hyperoxia only in males. Uptake of 18FDG was decreased after hyperoxia in the backbrain of females. Conclusion: We found that females at their reproductive senescence are more susceptible to hyperoxia, compared to males. We propose this model of hyperoxia as a useful tool to assess sex differences in adaptive response to acute stress conditions, which may be partially responsible for observed sex differences in longevity of CBA/H mice.
| Vrsta građe: | Rad objavljen u časopisu | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ključne riječi: | cell aging; fluorodeoxyglucose F18; hyperoxia; mice; inbred CBA; neuroimaging | ||||||||||||||||
| Područja: | BIOMEDICINA I ZDRAVSTVO > Temeljne medicinske znanosti | ||||||||||||||||
| Zavodi: | Zavod za eksperimentalnu fiziku Zavod za molekularnu medicinu |
||||||||||||||||
| Projekti: |
|
||||||||||||||||
| Pohranio: | Marijana Popović Hadžija | ||||||||||||||||
| Datum pohrane: | 02 Feb 2017 13:23 | ||||||||||||||||
| URI: | https://fulir.irb.hr/id/eprint/3363 | ||||||||||||||||
| DOI: | 10.12659/MSMBR.895356 |
Actions (login required)
 |
Pokaži zapis |
Altmetric
Altmetric
