Introducing UV-Vis spectroscopy in characterizing the surface of lipid bilayers: II. A potential to study the permeabilization of mixed lipid membranes by antimicrobial peptide anoplin

Krajnović, Vedrana; Jakas, Andreja; Bakarić, Danijela (2026) Introducing UV-Vis spectroscopy in characterizing the surface of lipid bilayers: II. A potential to study the permeabilization of mixed lipid membranes by antimicrobial peptide anoplin. In: Advances in Biomembranes and Lipid Self-Assembly. Elsevier. (In Press)

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Abstract

In this study, we showcased the effectiveness of UV-Vis spectroscopy for characterizing the thermotropic properties of mixed lipid bilayers composed of phosphatidylcholine (PC), phosphatidylglycerol (PG), and either stearic acid (SA) or linoleic acid (LA), in the presence of the antimicrobial peptide anoplin (ANP). We measured the phase transition temperatures of these lipid bilayers using differential scanning calorimetry (DSC) as the standard method, and temperature-dependent UV-Vis spectroscopy. The advantage of UV-Vis spectroscopy stems from its ability to detect events that DSC does not register. Additionally, it demonstrated greater sensitivity in identifying differences in lipid bilayer phase transition temperatures depending on whether SA or LA was present, as well as in assessing the impact of ANP on membrane fluidity. Supplemented by measurements of lipid bilayer sizes, the results of this study highlight the potential of UV-Vis spectroscopy in identifying structural changes in lipid bilayers associated with membrane permeabilization.

Item Type:	Book Section
Uncontrolled Keywords:	Mixed lipid membranes; Fatty acids (FAs); Anoplin (ANP); Temperature-dependent UV-Vis spectroscopy; Multivariate curve analysis; Permeabilization
Subjects:	NATURAL SCIENCES NATURAL SCIENCES > Chemistry NATURAL SCIENCES > Chemistry > Physical Chemistry
Divisions:	Division of Organic Chemistry and Biochemistry
Projects:	Project title Project leader Project code Project type Model demijelinizacije na molekulskoj skali pri fiziološkim i patološkim uvjetima-DEMYMOLSCALE Danijela Bakarić UIP-2020-02-7669 HRZZ Antimikrobni peptidi temeljeni na prirodnim morskim antimikrobicima: Dizajn i studija načina djelovanja Andreja Jakas IP-2024-05-1216 HRZZ
Depositing User:	Danijela Bakarić
Date Deposited:	03 Sep 2026 05:54
URI:	https://fulir.irb.hr:/id/eprint/12131
DOI:	10.1016/bs.abl.2026.07.003

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