Microbiome Profiling of Biofilms Formed on d-PTFE Membranes Used in Guided Bone Regeneration

Mohar Vitezić, Bojana; Franović, Barbara; Renko, Ira; Kuiš, Davor; Begić, Gabrijela; Blašković, Marko; Gabrić, Dragana; Nikolić, Marina; Šoić Vranić, Tamara; Veljanovska, Diana; Cvijanović Peloza, Olga (2025) Microbiome Profiling of Biofilms Formed on d-PTFE Membranes Used in Guided Bone Regeneration. Microorganisms, 13 (11). ISSN 2076-2607

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Abstract

In guided bone regeneration (GBR) procedures, d-PTFE membranes are often used as a barrier to promote alveolar ridge regeneration. The aim of this randomized clinical trial was to examine the microbial diversity and structure of biofilms on two types of d-PTFE membranes, Permamem® and Cytoplast™, over four-week oral cavity exposure periods. Bacterial biofilm analysis was performed using 16S rRNA next-generation sequencing (NGS) on 36 samples (20 Permamem® and 16 Cytoplast™). The results showed significant differences in the microbial profiles: Cytoplast™ membranes showed reduced microbial diversity and an enhanced proportion of pathobionts like Selenomonas, Segatella, Fusobacterium and Parvimonas, which are associated with periodontal and peri-implant diseases and alveolar bone loss. Permamem® membranes promoted colonization by bacteria associated with healthy oral conditions, such as the genera Streptococcus, Kingella and Corynebacterium. Overall, our results showed that Cytoplast™ membranes generate a specific type of biofilm, leading to reduction in health-related bacterial species and facilitating growth conditions for dysbiosis shift. Further research and patient follow-ups are essential to thoroughly evaluate the clinical implications of different d-PTFE membranes used in guided bone regeneration.

Item Type:	Article
Uncontrolled Keywords:	biofilm; microbiome; membrane; metagenomics
Subjects:	BIOMEDICINE AND HEALTHCARE
Divisions:	Division of Electronics
Projects:	Project title Project leader Project code Project type Usporedba reparacijskog odgovora koštanog tkiva upotrebom dentina, ksenogenog biomaterijala i autologne kosti-MgXBiomBone Olga Peloza; Davor Kuiš; Sonja Pezelj-Ribarić; Željka Perić Kačarević; Branko Trajkovski; Marko Blašković; Matko Oguić; Marija Pejakić IP-2020-02-7875 HRZZ
Depositing User:	Ana Zečević
Date Deposited:	05 May 2026 13:06
URI:	https://fulir.irb.hr:/id/eprint/11901
DOI:	10.3390/microorganisms13112478

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