p27 Expression in Wild‐Type KRAS Colon Cancer

Marinović, Sonja; Paladin, Iva; Škrtić, Anita; Catela Ivković, Tina; Verbanac, Donatella; Kapitanović, Sanja (2026) p27 Expression in Wild‐Type KRAS Colon Cancer. Journal of Cellular and Molecular Medicine, 30 (12). ISSN 1582-1838

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Abstract

p27, a cyclin-dependent kinase inhibitor, functions as a tumour suppressor in the nucleus but may acquire oncogenic properties when mislocalized to the cytoplasm. While KRAS mutations can induce p27 phosphorylation and cytoplasmic retention, the regulation and significance of p27 expression in wild-type (WT) KRAS colorectal cancer (CRC) remain unclear. This study investigated the relationship between WT KRAS status and p27 localization, as well as the potential roles of miR-221/222 expression and the CDKN1B V109G polymorphism in CRC susceptibility. Immunohistochemical analysis of 50 WT KRAS CRCs and adjacent normal tissues revealed the highest percentage of p27-positive cells in the superficial layer of normal mucosa and significantly fewer in the tumour center. WT KRAS tumours with KRAS expression showed increased p27 expression and predominant cytoplasmic localization at the invasive front, suggesting altered p27 subcellular distribution. miR-221/222 expression showed no correlation with p27 levels, and the CDKN1B V109G polymorphism was not associated with CRC risk. This study is the first to examine p27 localization in WT KRAS CRC. The observed association between WT KRAS expression and cytoplasmic p27 localization highlights a potential mechanism contributing to tumour progression through altered p27 function.

Item Type:	Article
Uncontrolled Keywords:	colon adenocarcinoma; KRAS; miR-221/222; p27; V109G SNP
Subjects:	BIOMEDICINE AND HEALTHCARE
Divisions:	Division of Molecular Medicine
Projects:	Project title Project leader Project code Project type Mikrosatelitna nestabilnost (MSI i EMAST) u molekularnom profiliranju sporadičnih karcinoma debelog crijeva-MSIandEMASTinCRC Sanja Kapitanović IP-2016-06-1430 HRZZ
Depositing User:	Ivana Vuglec
Date Deposited:	16 Jul 2026 12:28
URI:	https://fulir.irb.hr:/id/eprint/12092
DOI:	10.1111/jcmm.71248

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