Anoplin-functionalized gold nanoparticles cause greater disorder in model bacterial membranes compared to individual components

(COGITO collaboration) Krajnović, Vedrana; Bosurgi, Emanuele; Jakas, Andreja; Čikoš, Ana; Edely, Mathieu; de la Chapelle, Marc Lamy; Bakarić, Danijela (2026) Anoplin-functionalized gold nanoparticles cause greater disorder in model bacterial membranes compared to individual components. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 363 . ISSN 1386-1425

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Abstract

Antimicrobial peptides are vital to the innate immune system and serve as a safe alternative to traditional antibiotics for bacterial infections. However, their effectiveness is limited by the need for high concentrations to disrupt bacterial membranes and the challenge of protecting them from proteases that degrade them before reaching their target. Intending to improve the delivery of anoplin (ANP) to bacterial cells, we prepared ANP-coated gold nanoparticles (Au NPs) with a diameter of about 50 nm, and investigated their effects on the properties of model bacterial membranes using Raman and surface-enhanced Raman spectroscopy (SERS). Prepared as a mixture of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(1′-rac-glycerol) sodium salt (PG) at a ratio PE/PG 3/1, lipid membranes were measured in the absence/presence of pure ANP, Au NPs (coated only with citrate), and Au NPs coated with ANP and at two different temperatures, 10 °C and 26 °C, at which the lipids are found in the gel/fluid and fluid/fluid phases, respectively. In addition to serving as indicators of the disorder caused by the phase change in pure PE/PG bilayers, the bands of skeletal vibrations (νC-Ctrans/gauche) proved to be sensitive enough to detect the increased disorder resulting from the adsorption of ANP and of ANP-coated Au NPs. Since the disorder caused by the adsorption of the latter is significantly greater than that caused by ANP itself, we believe that this study could pave new ways in the research and use of Au NPs (as well as other metal NPs) as targeted and effective delivery of therapeutics.

Item Type:	Article
Uncontrolled Keywords:	Anoplin (ANP: GLLKRIKTLL-NH2); Model lipid membranes; Gold nanoparticles (Au NPs); Surface-enhanced Raman spectroscopy (SERS)
Subjects:	NATURAL SCIENCES NATURAL SCIENCES > Chemistry NATURAL SCIENCES > Chemistry > Physical Chemistry
Divisions:	Division of Organic Chemistry and Biochemistry
Projects:	Project title Project leader Project code Project type Model demijelinizacije na molekulskoj skali pri fiziološkim i patološkim uvjetima-DEMYMOLSCALE Danijela Bakarić UIP-2020-02-7669 HRZZ Antimikrobni peptidi temeljeni na prirodnim morskim antimikrobicima: Dizajn i studija načina djelovanja Andreja Jakas IP-2024-05-1216 HRZZ Metaproteomika patvorenih namirnica Mario Cindrić NPOO.C3.2.R3-I1.04.0122 EU
Depositing User:	Danijela Bakarić
Date Deposited:	13 Jul 2026 07:19
URI:	https://fulir.irb.hr:/id/eprint/12038
DOI:	10.1016/j.saa.2026.128379

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