Ortho-substituted PCB 153: effects in CHO-K1 cells

Miletić, Marina; Murati, Teuta; Šimić, Branimir; Bilandžić, Nina; Brozović, Anamaria; Kmetič, Ivana (2021) Ortho-substituted PCB 153: effects in CHO-K1 cells. Archives of Industrial Hygiene and Toxicology, 72 (4). pp. 326-332. ISSN 1848-6312

PDF - Published Version - article
Available under License Creative Commons Attribution Non-commercial No Derivatives.
Download (1MB)

Official URL: http://doi.org/10.2478/aiht-2021-72-3588

Abstract

Non-planar di-ortho-substituted PCB 153 (2,2',4,4',5,5'-hexachlorobiphenyl), one of the most abundant PCB congeners in the environment and in biological and human tissues, has been identified as potential endocrine disruptor affecting the reproductive and endocrine systems in rodents, wildlife, and humans. The aim of this study was to gain a deeper insight into its mode/mechanism of action in Chinese hamster ovary K1 cells (CHO-K1). PCB 153 (10-100 mu mol/L) inhibited CHO-K1 cell proliferation, which was confirmed with four bioassays (Trypan Blue, Neutral Red, Kenacid Blue, and MTT), of which the MTT assay proved the most sensitive. PCB 153 also induced ROS formation in a dose-dependent manner. Apoptosis was seen after 6 h of exposure to PCB 153 doses >= 50 mu mol/L, while prolonged exposure resulted in the activation of the necrotic pathway. PCB 153-induced disturbances in normal cell cycle progression were time-dependent, with the most significant effects occurring after 72 h.

Item Type:

Article

Uncontrolled Keywords:

cell cycle progression ; cell death ; cytotoxicity ; polychlorinated biphenyls ; ROS

Subjects:

BIOTECHNICAL SCIENCES > Biotechnology
BIOTECHNICAL SCIENCES > Interdisciplinary Technical Sciences
INTERDISCIPLINARY AREAS OF KNOWLEDGE > Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area

Divisions:

Division of Molecular Biology

Projects: