Rotavirus A in Domestic Pigs and Wild Boars: High Genetic Diversity and Interspecies Transmission

Brnić, Dragan; Čolić, Daniel; Kunić, Valentina; Maltar-Strmečki, Nadica; Krešić, Nina; Konjević, Dean; Bujanić, Miljenko; Bačani, Ivica; Hižman, Dražen; Jemeršić, Lorena (2022) Rotavirus A in Domestic Pigs and Wild Boars: High Genetic Diversity and Interspecies Transmission. Viruses, 14 (9). ISSN 1999-4915

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Abstract

Rotavirus A (RVA) is an important pathogen for porcine health. In comparison to humans, RVA in domestic animals and especially in wildlife is under researched. Therefore, the aim of the present study was to investigate the prevalence, genetic diversity, molecular epidemiology and inter-species transmission of RVA in domestic pigs and wild boars. During the three consecutive RVA seasons (2018–2021) we collected 445 and 441 samples from domestic pigs and wild boars, respectively. Samples were tested by real-time RT- PCR, and RVA-positive samples were genotyped in VP7 and VP4 segments. Our results report an RVA prevalence of 49.9% in domestic pigs and 9.3% in wild boars. Outstanding RVA genetic diversity was observed in VP7 and VP4 segments, especially in domestic pigs exhibiting a striking 23 different RVA combinations (G5P[13] and G9P[23] prevailed). Interspecies transmission events were numerous between domestic pigs and wild boars, sharing G3, G5, G6, G9, G11 and P[13] genotypes. Furthermore, our data indicate that such transmission events involved even bovines (G6, P[11]) and, intriguingly, humans (G1P[8]). This study contributes to the basic knowledge that may be considered important for vaccine development and introduction, as a valuable and currently missing tool for efficient pig health management in the EU.

Item Type:	Article
Uncontrolled Keywords:	rotavirus A ; domestic pig ; wild boar ; genotype ; genetic diversity ; molecular epidemiology ; phylogenetic analysis ; interspecies transmission ; Croatia
Subjects:	BIOMEDICINE AND HEALTHCARE > Veterinary Medicine
Divisions:	Division of Physical Chemistry
Projects:	Project title Project leader Project code Project type Rotavirusi u ekosustavu Republike Hrvatske: molekularna epidemiologija i zoonotski potencijal Brnić, Dragan UIP-2017-05-8580 HRZZ
Depositing User:	Nadica Maltar Strmečki
Date Deposited:	04 Oct 2022 12:32
URI:	http://fulir.irb.hr/id/eprint/7569
DOI:	10.3390/v14092028

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