An outbreak of ertapenem resistant, carbapenemase-negative and porin-deficient ESBL-producing Klebsiella pneumoniae complex

Matovina, Mihaela; Abram, Maja; Repac-Antić, Davorka; Knežević, Samira; Bubonja-Šonje, Marina (2021) An outbreak of ertapenem resistant, carbapenemase-negative and porin-deficient ESBL-producing Klebsiella pneumoniae complex. GERMS, 11 (2). pp. 199-210. ISSN 2248-2997

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Abstract

Introduction: Carbapenem-resistant Klebsiella pneumoniae is an emerging healthcare-associated pathogen with dynamic molecular epidemiology. This study presents a retrospective analysis of the distribution and antibiotic resistance patterns of ertapenem-resistant ESBL-producing K. pneumoniae strains recovered during an outbreak from 2012 to 2014 in a Croatian University hospital. Methods We aimed to estimate genetic relatedness of clinical isolates and underlying mechanisms that conferred the ertapenem- resistant phenotype. Results: Expression analysis of genes involved in the antibiotic resistance showed reduced expression of major non-selective porin channel OmpK35. Reduced expression of OmpK36 porin channel in isolates resistant to at least one more carbapenem, apart from the ertapenem, was found to a lesser degree. Pulsed-field gel electrophoresis analysis of genomic DNA revealed that almost all isolates belonged to the same genetic clone. Conclusions: Caution regarding ertapenem- resistant, carbapenemase-negative porin- deficient mutants of K. pneumoniae is required as they are widespread, and under selective pressure this could result in a local clonal outbreak.

Item Type:	Article
Uncontrolled Keywords:	ertapenem ; drug resistance ; Klebsiella pneumoniae ; molecular epidemiology ; outbreak
Subjects:	BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences
Divisions:	Division of Organic Chemistry and Biochemistry
Projects:	Project title Project leader Project code Project type Proširenost i klinički značaj HBs antigenih mutanti virusa hepatitisa B Bubonja Šonje, Marina uniri-biomed-18-215 NadSve Bakterijska patogeneza - od istraživanja do kliničke primjene- Maja Abram uniri-biomed-18-277 NadSve
Depositing User:	Mihaela Matovina
Date Deposited:	25 Aug 2021 07:42
URI:	http://fulir.irb.hr/id/eprint/6536
DOI:	10.18683/germs.2021.1257

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