Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen

Štambuk, Nikola; Konjevoda, Paško; Turčić, Petra; Šošić, Hrvoje; Aralica, Gorana; Babić, Damir; Seiwerth, Sven; Kaštelan, Željko; Novak Kujundžić, Renata; Wardega, Piotr; Barać Žutelija, Jelena; Gudelj Gračanin, Ana; Gabričević, Mario (2019) Targeting Tumor Markers with Antisense Peptides: An Example of Human Prostate Specific Antigen. International Journal of Molecular Sciences, 20 (9). ISSN 1661-6596

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Official URL: https://www.mdpi.com/1422-0067/20/9/2090

Abstract

The purpose of this paper was to outline the development of short peptide targeting of the human prostate specific antigen (hPSA), and to evaluate its effectiveness in staining PSA in human prostate cancer tissue. The targeting of the hPSA antigen by means of antisense peptide AVRDKVG was designed according to a three-step method involving: 1. The selection of the molecular target (hPSA epitope), 2. the modeling of an antisense peptide (paratope) based on the epitope sequence, and 3. the spectroscopic evaluation of sense–antisense peptide binding. We then modified standard hPSA immunohistochemical staining practice by using a biotinylated antisense peptide instead of the standard monoclonal antibody and compared the results of both procedures. Immunochemical testing on human tissue showed the applicability of the antisense peptide technology to human molecular targets. This methodology represents a new approach to deriving peptide ligands and potential lead compounds for the development of novel diagnostic substances, biopharmaceuticals and vaccines.

Item Type:

Article

Uncontrolled Keywords:

neoplasm ; prostate specific antigen ; biomarker ; antisense peptide ; immunohistochemistry ; nanomedicine

Subjects:

NATURAL SCIENCES > Interdisciplinary Natural Sciences

Divisions:

NMR Center

Projects: