Structural analysis of brain ganglioside acetylation patterns in mice with altered ganglioside biosynthesis

Mlinac, Kristina; Fabris, Dragana; Vukelić, Željka; Rožman, Marko; Heffer, Marija; Kalanj Bognar, Svjetlana (2013) Structural analysis of brain ganglioside acetylation patterns in mice with altered ganglioside biosynthesis. Carbohydrate Research, 382 . pp. 1-8. ISSN 0008-6215

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Abstract

Gangliosides are sialylated membrane glycosphingolipids especially abundant in mammalian brain tissue. Sialic acid O-acetylation is one of the most common structural modifications of gangliosides which considerably influences their chemical properties. In this study, gangliosides extracted from brain tissue of mice with altered ganglioside biosynthesis (St8sia1 null and B4galnt1 null mice) were structurally characterized and their acetylation pattern was analyzed. Extracted native and alkali-treated gangliosides were resolved by high performance thin layer chromatography. Ganglioside mixtures as well as separated individual ganglioside fractions were further analyzed by tandem mass spectrometry. Several O-acetylated brain ganglioside species were found in knockout mice, not present in the wild-type mice. To the best of our knowledge this is the first report on the presence of O-acetylated GD1a in St8sia1 null mice and O-acetylated GM3 species in B4galnt1 null mice. In addition, much higher diversity of abnormally accumulated brain ganglioside species regarding the structure of ceramide portion was observed in knockout versus wild-type mice. Obtained findings indicate that the diversity of brain ganglioside structures as well as acetylation patterns in mice with altered ganglioside biosynthesis, is even higher than previously reported. Further investigation is needed in order to explore the effects of acetylation on ganglioside interactions with other molecules and consequently the physiological role of acetylated ganglioside species.

Item Type:

Article

Uncontrolled Keywords:

brain gangliosides; mass spectrometric characterization; O-acetylated ganglioside species; O-acetylation patterns; St8sia1 null and B4galnt1 null mice

Subjects:

NATURAL SCIENCES > Chemistry

Divisions:

Division of Physical Chemistry

Projects:

Project title	Project leader	Project code	Project type
Spektroskopija, kemijska svojstva i reakcije biološki aktivnih molekula-	Branka Kovač	098-0982915-2945	MZOS
Uloga lipidnih splavi i glikokonjugata u razvoju i regeneraciji živčanog sustava-	Marija Heffer	219-0061194-2158	MZOS
Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije-	Željka Vukelić	108-1081870-2415	MZOS
Uloga membranskih lipida u moždanom razvitku, starenju i neurodegeneraciji-	Svjetlana Kalanj-Bognar	108-1081870-1877	MZOS