Mechanisms of carbapenem resistance in multi drug resistant clinical isolates of Pseudomonas aeruginosa from a Croatian hospital

Bubonja-Šonje, Marina; Matovina, Mihaela; Škrobonja, Ivana; Bedenić, Branka; Abram, Maja (2015) Mechanisms of carbapenem resistance in multi drug resistant clinical isolates of Pseudomonas aeruginosa from a Croatian hospital. Microbial Drug Resistance, 21 (3). pp. 261-269. ISSN 1076-6294

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Abstract

Pseudomonas aeruginosa is opportunistic pathogen, one of the leading causes of nosocomial infections such as pneumonia, urinary tract infections, and bacteraemia. The bacterial resistance to structurally unrelated antibiotics and its spread within hospitals limits the efficient antimicrobial options and patients' outcome. Carbapenems are important agents for the therapy of infections due to multidrug-resistant (MDR) P. aeruginosa, hence the development of carbapenem resistance severely hampers effective therapeutic options. The aim of this investigation was to examine mechanisms of carbapenem resistance and genomic diversity in carbapenem-resistant MDR strains of P. aeruginosa which caused an outbreak among patients in Clinical Hospital Rijeka. Most of the isolates showed decreased expression of porin that is important for the entry of carbapenems (oprD). Overexpression of MexAB-OprM, MexCD-OprJ, and MexEF-OprN efflux systems was observed in many of the isolates. Production of metallo-beta-lactamases (MBL) was not detected. Typing by pulsed-field gel electrophoresis discriminated the isolates into five clusters. The clonal distribution of the strains was related to the location of hospital departments where the isolates were collected, which implies that most of the infections were caused by spread of the epidemic strains within the hospital.

Item Type:	Article
Additional Information:	This work was supported by ‘‘Molecular mechanisms of bacterial pathogenesis and response to stress’’ (M.A., grant number 13.06.1.1.07. from the University of Rijeka).
Uncontrolled Keywords:	antimicrobial resistance; carbapenems; efflux pumps; porins; Pseudomonas aeruginosa
Subjects:	BIOMEDICINE AND HEALTHCARE > Clinical Medical Sciences
Divisions:	Division of Organic Chemistry and Biochemistry
Depositing User:	Mihaela Matovina
Date Deposited:	08 Dec 2016 09:54
URI:	http://fulir.irb.hr/id/eprint/2664
DOI:	10.1089/mdr.2014.0172

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