Sub-Emetic Toxicity of Bacillus cereus Toxin Cereulide on Cultured Human Enterocyte-Like Caco-2 Cells.

Rajkovic, Andreja; Grootaert, Charlotte; Butorac, Ana; Cucu, Tatiana; De Meulenaer, Bruno; Van Camp, John; Bracke, Marc; Uyttendaele, Mieke; Bačun-Družina, Višnja; Cindrić, Mario (2014) Sub-Emetic Toxicity of Bacillus cereus Toxin Cereulide on Cultured Human Enterocyte-Like Caco-2 Cells.. Toxins, 6 (8). pp. 2270-2290. ISSN 2072-6651

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Abstract

Cereulide (CER) intoxication occurs at relatively high doses of 8 µg/kg body weight. Recent research demonstrated a wide prevalence of low concentrations of cereulide in rice and pasta dishes. However, the impact of an exposure to low doses of cereulide was never studied before. In this research, we investigate the effect of low concentrations of cereulide on the behavior of intestinal cells using Caco-2 cell line. The MTT (mitochondrial 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and the SRB (Sulforhodamine B) reactions were used to measure the mitochondrial activity and the measurement of cellular protein content, respectively. Both assays showed that differentiated Caco-2 cells were sensitive to low concentrations of CER (in MTT reaction 1 ng/mL after 3 days of treatment ; in SRB reaction 0.125 ng/mL after 3 days of treatment). Cell counts revealed that cells were released from the differentiated monolayer at 0.5 ng/mL of cereulide. Additionally, 0.5 and 2 ng/mL of CER increased lactate presence in the cell culture medium. Proteomic data showed that cereulide at concentration of 1 ng/mL led towards a significant decrease in energy managing and H2O2 detoxification proteins and to an increase in cell death markers. This is amongst first reports to describe the influence of sub-emetic concentrations of cereulide on a differentiated intestinal monolayer model showing that low doses may induce an altered enterocyte metabolism and membrane integrity.

Item Type:	Article
Uncontrolled Keywords:	Bacillus cereus ; cereulide ; emetic toxin ; doses ; cell ; toxicity ; differentiated Caco-2
Subjects:	BIOTECHNICAL SCIENCES > Biotechnology
Divisions:	Division of Molecular Medicine
Projects:	Project title Project leader Project code Project type Stress-induced diversity and evolution of mixed bacterial cultures (Stresom izazvana raznolikost i evolucija mješovitih bakterijskih kultura)- Višnja Bačun-Družina 058-0583444-3466 MZOS
Depositing User:	Mario Cindrić
Date Deposited:	01 Oct 2015 12:33
URI:	http://fulir.irb.hr/id/eprint/2145
DOI:	10.3390/toxins6082270

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