Primary DNA damage assessed with the comet assay and comparison to the absorbed dose of diagnostic X-rays in children

Milković, Đurđica; Garaj-Vrhovac, Vera; Ranogajec-Komor, Maria; Miljanić, Saveta; Gajski, Goran; Knežević, Željka; Beck, Natko (2009) Primary DNA damage assessed with the comet assay and comparison to the absorbed dose of diagnostic X-rays in children. International Journal of Toxicology, 28 (5). pp. 405-416. ISSN 1091-5818

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Abstract

The aim of this work is to assess DNA damage in peripheral blood lymphocytes of children prior to and following airway X-ray examinations of the chest using the alkaline comet assay and to compare data with the measured absorbed dose. Twenty children with pulmonary diseases, between the ages of 5 and 14 years, are assessed. Absorbed dose measurements are conducted for posterior-anterior projection on the forehead, thyroid gland, gonads, chest, and back. Doses are measured using thermoluminescent and radiophotoluminescent dosimetry systems. Differences between tail lengths, tail intensity, and tail moments as well as for the long-tailed nuclei before and after exposures are statistically significant and are dependent on the individual. The results demonstrate the usefulness of the comet assay as a measure of X-ray damage to lymphocytes in a clinical setting. Doses measured with both dosimeters show satisfactory agreement (0.01 mSv) and are suitable for dosimetric measurements in X-ray diagnostics.

Item Type:	Article
Uncontrolled Keywords:	x-ray dosimetry; DNA damage; human peripheral blood lymphocytes; alkaline comet assay; ALARA
Subjects:	NATURAL SCIENCES > Interdisciplinary Natural Sciences > Radiation Science
Divisions:	Division of Materials Chemistry
Projects:	Project title Project leader Project code Project type Fizičko-kemijski učinci ionizirajućih zračenja u materijalima- Branka Mihaljević 098-0982904-2954 MZOS Učinci radioloških dijagnostičkih postupaka pri pregledu dišnih putova u djece- Đurđica Milković 277-2770966-0962 MZOS Mutageni i antimutageni u ekogenetičkim istraživanjima- Vera Garaj-Vrhovac 022-0222148-2125 MZOS
Depositing User:	Željka Knežević Medija
Date Deposited:	22 Dec 2016 11:24
URI:	http://fulir.irb.hr/id/eprint/1836
DOI:	10.1177/1091581809344775

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