Teucrium montanum L.—Unrecognized Source of Phenylethanoid Glycosides: Green Extraction Approach and Elucidation of Phenolic Compounds via NMR and UHPLC-HR MS/MS

Mandura Jarić, Ana; Čikoš, Ana; Pocrnić, Marijana; Aladić, Krunoslav; Jokić, Stela; Šeremet, Danijela; Vojvodić Cebin, Aleksandra; Komes, Draženka (2023) Teucrium montanum L.—Unrecognized Source of Phenylethanoid Glycosides: Green Extraction Approach and Elucidation of Phenolic Compounds via NMR and UHPLC-HR MS/MS. Antioxidants, 12 (11). ISSN 2076-3921

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Abstract

Health-oriented preferences, a demand for innovative food concepts, and technological advances have greatly influenced changes in the food industry and led to remarkable development of the functional food market. Incorporating herbal extracts as a rich source of bioactive compounds (BC) could be an effective solution to meet the high demand of consumers in terms of expanding the high-quality range of functional foods. The aim of this study is the valorization of the bioactive potential of T. montanum L., an understudied Mediterranean plant species, and the in-depth elucidation of a polyphenolic profile with a UHPLC-HR MS/MS and NMR analysis. The total phenolic content (TPC) and antioxidant capacity (AC) were determined on heat-assisted (HAE), microwave-assisted (MAE) and subcritical water (SWE) extracts. In terms of antioxidant capacity, SWE extracts showed the most notable potential (ABTS: 0.402–0.547 mmol eq Trolox g−1 dw, DPPH: 0.336–0.427 mmol eq Trolox g−1 dw). 12 phenolic compounds were identified in the samples of T. montanum from six microlocations in Croatia, including nine phenylethanoid glycosides (PGs) with total yields of 30.36–68.06 mg g−1 dw and 25.88–58.88 mg g−1 dw in HAE and MAE extracts, respectively. Echinacoside, teupolioside, stachysoside A, and poliumoside were the most abundant compounds HAE and MAE extracts, making T. montanum an emerging source of PGs.

Item Type:	Article
Uncontrolled Keywords:	microwave-assisted extraction; phenylethanoid glycosides; polyphenols; subcritical water extraction; Teucrium montanum L.
Subjects:	NATURAL SCIENCES > Chemistry > Analytic Chemistry
Divisions:	NMR Center
Projects:	Project title Project leader Project code Project type Formuliranje inkapsuliranih sustava bioaktivnih sastojaka tradicionalnih biljnih vrsta: trave ive i dobričice namijenjenih razvoju inovativnih funkcionalnih prehrambenih proizvoda-FUNCBIOCAP Draženka Komes; Krunoslav Žižek; Vesna Benković; Sofia Ana Blažević; Anica Horvat Knežević; Arijana Martinić; Joško Barbarić IP-2019-04-5879 HRZZ
Depositing User:	Ana Čikoš
Date Deposited:	21 Jan 2025 15:18
URI:	http://fulir.irb.hr/id/eprint/9499
DOI:	10.3390/antiox12111903

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