Binding of Glutamic Acid to Silver and Gold Nanoparticles Investigated by Surface-Enhanced Raman Spectroscopy

Mohaček-Grošev, Vlasta; Škrabić, Marko; Gebavi, Hrvoje; Blažek Bregović, Vesna; Marić, Ivan; Amendola, Vincenzo; Grdadolnik, Jože (2024) Binding of Glutamic Acid to Silver and Gold Nanoparticles Investigated by Surface-Enhanced Raman Spectroscopy. Biosensors, 14 (11). ISSN 2079-6374

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Abstract

Glutamate is the most important excitatory neurotransmitter, which is relevant for the study of several diseases such as amyotrophic lateral sclerosis and Alzheimer. It is the form L-glutamic acid (Glu) takes at physiologically relevant pHs. The surface-enhanced Raman spectra of Glu obtained at pH values ranging from 3.3 to 12 are collected in the presence of silver and gold colloids and on solid substrates. The observed bands are compared with the positions of calculated normal modes for free neutral glutamic acid, glutamic acid monohydrate, glutamic acid bound to gold and silver atoms, and sodium glutamate. Although gold atoms prefer to bind to the NH2 group as compared to carbonyl groups, silver atoms prefer binding to hydroxyl groups more than binding to the amino group. SERS spectra of glutamic acid solutions with a pH value of 12, in which both carboxylic groups are deprotonated, indicate a complexation of the glutamic acid dianion with the sodium cation, which was introduced into the solution to adjust the pH value. Further research towards an optimal substrate is needed.

Item Type:	Article
Uncontrolled Keywords:	glutamate; glutamic acid; surface-enhanced Raman spectroscopy; AuNP; AgNP; CRYSTAL09; normal modes
Subjects:	NATURAL SCIENCES > Physics > Atomic and Molecular Physics NATURAL SCIENCES > Chemistry > Physical Chemistry
Divisions:	Division of Materials Chemistry Division of Materials Physics Center of Excellence for Advanced Materials and Sensing Devices
Projects:	Project title Project leader Project code Project type Proučavanje vezanja bioloških molekula na supstrat metodom površinski pojačanog Ramanovog raspršenja (SERS) i površinski pojačane infracrvene spektroskopije (SEIRS)- Vlasta Mohaček-Grošev N/A MZO
Depositing User:	Lorena Palameta
Date Deposited:	29 Oct 2024 10:03
URI:	http://fulir.irb.hr/id/eprint/9208
DOI:	10.3390/bios14110522

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