Bacterial hosts of clinically significant beta-lactamase genes in Croatian wastewaters

Dekić Rozman, Svjetlan; Puljko, Ana; Karkman, Antti; Virta, Marko; Udiković-Kolić, Nikolina (2024) Bacterial hosts of clinically significant beta-lactamase genes in Croatian wastewaters. FEMS Microbiology Ecology, 100 (6). ISSN 1574-6941

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Abstract

Wastewater treatment plants (WWTPs) provide a suitable environment for the interaction of antibiotic resistant bacteria and antibiotic-resistance genes (ARGs) from human, animal, and environmental sources. The aim was to study the influent and effluent of two WWTPs in Croatia to identify bacterial hosts of clinically important beta-lactamase genes (blaTEM, blaVIM, blaOXA-48-like) and observe how their composition changes during the treatment process. A culture-independent epicPCR (Emulsion, Paired isolation and Concatenation Polymerase Chain Reaction) was used to identify the ARG hosts, and 16S rRNA amplicon sequencing to study the entire bacterial community. Different wastewater sources contributed to the significant differences in bacterial composition of the wastewater between the two WWTPs studied. A total of 167 genera were detected by epicPCR, with the Arcobacter genus, in which all ARGs studied were present, dominating in both WWTPs. In addition, the clinically important genera Acinetobacter and Aeromonas contained all ARGs examined. The blaOXA-48-like gene had the highest number of hosts, followed by blaVIM, while blaTEM had the narrowest host range. Based on 16S rRNA gene sequencing, ARG hosts were detected in both abundant and rare taxa. The number of hosts carrying investigated ARGs was reduced by wastewater treatment. EpicPCR provided valuable insights into the bacterial hosts of horizontally transmissible beta-lactamase genes in Croatian wastewater.

Item Type:	Article
Uncontrolled Keywords:	16S rRNA; antibiotic-resistance genes; bacteria; Croatia; epicPCR; wastewater
Subjects:	NATURAL SCIENCES > Biology > Biochemistry and Molecular Biology NATURAL SCIENCES > Interdisciplinary Natural Sciences BIOTECHNICAL SCIENCES > Interdisciplinary Technical Sciences
Divisions:	Division for Marine and Enviromental Research
Projects:	Project title Project leader Project code Project type Antibiotička rezistencija u uređajima za obradu otpadnih voda u Hrvatskoj: naglasak na ß-laktamaze proširenog spektra i karbapenemaze-WasteCare Nikolina Udiković Kolić; Stela Križanović; Ana Maravić; Marko Jelić; Josipa Kosić-Vukšić; Tamara Jurina; Marko Virta; Gisle Vestergaard IP-2019-04-5539 HRZZ
Depositing User:	Ana Puljko
Date Deposited:	24 Oct 2024 07:06
URI:	http://fulir.irb.hr/id/eprint/9187
DOI:	10.1093/femsec/fiae081

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