A new method for detection of HNE-histidine conjugates in rat inflammatory cells

Živković, Morana; Žarković, Kamelija; Škrinjar, Ljubomir; Georg, Waeg; Poljak-Blaži, Marija; Borović Šunjić, Suzana; Schaur, Rudolf Jörg; Žarković, Neven (2005) A new method for detection of HNE-histidine conjugates in rat inflammatory cells. Croatica Chemica Acta, 78 (1). pp. 91-98. ISSN 0011-1643

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Abstract

Oxidative stress, excessive production of reactive oxygen species, is considered an important part of different disorders, as well as of physiological processes (inflammation). The difference between physiological and pathological oxidative stress is often the occurrence of lipid peroxidation and its final toxic products, among which is 4-hydroxy-2-nonenal (HNE), a reactive aldehyde that forms protein conjugates. The aim of this study was to determine the distribution of HNE-histidine conjugates in leukocytes during systemic inflammation. We used genuine monoclonal antibodies against HNE-histidine conjugates for immuno-cytochemical, immuno-histochemical and immuno-electron microscopical analyses of HNE in inflammatory cells. Spleen tissue, leukocytes from blood and macrophages from the peritoneum of rats intraperitoneally (i.p.) injected with micronized zeolite (MZ) were analyzed. HNE-histidine conjugates were predominantly detected near cell membranes, phagosomes and macrophage granules. Immunodetection of HNE-histidine conjugates may be used as an analytical immunochemical method to study HNE formation in pathological and physiological processes and for pathomorphological diagnostic procedures.

Item Type:	Article
Uncontrolled Keywords:	4-hydroxy-2-nonenal; oxidative stress; leukocytes; immunogold method; lipid-peroxidation product; nervous-system trauma; zeolite clinoptilolite; oxidative stress; end-product; growth; cells; proteins; involvement; anticancer
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	Division of Molecular Medicine
Projects:	Project title Project leader Project code Project type Oksidativni stres i zloćudne bolesti Neven Žarković 0098101 MZOS Infektivne bolesti i oksidativni stres središnjeg živčanog sustava Kamelija Žarković 0108298 MZOS
Depositing User:	Neven Žarković
Date Deposited:	08 Nov 2013 10:36
URI:	http://fulir.irb.hr/id/eprint/905

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