Membrane Fluctuation Model for Understanding the Effect of Receptor Nanoclustering on the Activation of Natural Killer Cells through Biomechanical Feedback

Pandey, Ashish; Nowakowski, Piotr; Ureña Martin, Carlos; Abu Ahmad, Muhammad; Edri, Avishay; Toledo, Esti; Tzadka, Sivan; Walther, Jonas; Le Saux, Guillaume; Porgador, Angel; Smith, Ana-Sunčana; Schvartzman, Mark (2024) Membrane Fluctuation Model for Understanding the Effect of Receptor Nanoclustering on the Activation of Natural Killer Cells through Biomechanical Feedback. Nano Letters, 24 (18). pp. 5395-5402. ISSN 1530-6984

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Abstract

We investigated the role of ligand clustering and density in the activation of natural killer (NK) cells. To that end, we designed reductionist arrays of nanopatterned ligands arranged with different cluster geometries and densities and probed their effects on NK cell activation. We used these arrays as an artificial microenvironment for the stimulation of NK cells and studied the effect of the array geometry on the NK cell immune response. We found that ligand density significantly regulated NK cell activation while ligand clustering had an impact only at a specific density threshold. We also rationalized these findings by introducing a theoretical membrane fluctuation model that considers biomechanical feedback between ligand–receptor bonds and the cell membrane. These findings provide important insight into NK cell mechanobiology, which is fundamentally important and essential for designing immunotherapeutic strategies targeting cancer.

Item Type:	Article
Additional Information:	PMID: 38684070
Uncontrolled Keywords:	natural killer cells; nanolithography; activation clustering; immune response
Subjects:	NATURAL SCIENCES > Physics > Condensed Matter Physics
Divisions:	Division of Physical Chemistry
Depositing User:	Ana Sunčana Smith
Date Deposited:	12 Jun 2024 10:45
URI:	http://fulir.irb.hr/id/eprint/8886
DOI:	10.1021/acs.nanolett.3c02815

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