Alpha-melanocyte stimulating hormone reduces colonic damage in rat model of inflammatory bowel disease

Tješić Drinković, Duška; Tješić Drinković, Dorian; Štambuk, Nikola; Konjevoda, Paško; Votava Raić, Ana; Vinković, Marijana; Vikić-Topić, Dražen (2005) Alpha-melanocyte stimulating hormone reduces colonic damage in rat model of inflammatory bowel disease. Croatica Chemica Acta, 78 (4). pp. 535-539. ISSN 0011-1643

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Abstract

The aim of the study was to investigate the dose-dependent in vivo effect of alpha-melanocyte stimulating hormone (alpha-MSH) in a rat model of inflammatory bowel disease induced by 2,4,6-trinitrobenzene sulphonic acid (TNBS). Laboratory animals (male Wistar rats weighing 200-250 g) were given 3 doses of alpha-MSH (0.5 mg/kg, I mg/kg, 2 mg/kg) intraperitoneally I hour prior to inducing colitis with a TNBS enema. Control animals received saline solution i.p. Rats were sacrificed after 72 hours and the area of mucosal lesions, involving the distal 10 cm of colon, was determined in mm(2) by means of image analysis software. a-MSH structure was analyzed by means of NMR spectroscopy. The area of colonic damage was significantly reduced following pretreatment with a single dose of I mg/kg a-MSH, as compared to control animals (p = 0.0147). Higher and lower doses had no significant effects. A single dose of I mg/kg a-MSH provided strong, statistically significant and pharmacologically relevant cytoprotection. The results point to a-MSH effectiveness in controlling inflammation and imply that further in vitro and in vivo experiments should be carried out to judge the importance and relevance of a-MSH in the control of inflammatory bowel disease. NMR data support a hairpin loop conformation of a-MSH in water solution, which includes conserved message sequence.

Item Type:	Article
Uncontrolled Keywords:	inflammatory bowel disease; alpha-MSH; NMR; TNBS; colitis; cytoprotection; MSH; receptors; colitis; peptide
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	NMR Center
Projects:	Project title Project leader Project code Project type Nuklearna magnetska rezonancija i proračuni bioorganskih molekula Dražen Vikić-Topić 0098059 MZOS Moduliranje imunološkog odgovora bioaktivnim peptidima Biserka Pokrić 0098097 MZOS
Depositing User:	Nikola Štambuk
Date Deposited:	30 Oct 2013 14:38
URI:	http://fulir.irb.hr/id/eprint/876

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