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Beginners guide to sample preparation techniques for transmission electron microscopy

Dumančić, Ena; Vojta, Lea; Fulgosi, Hrvoje (2023) Beginners guide to sample preparation techniques for transmission electron microscopy. Periodicum Biologorum, 125 (1-2). pp. 123-131. ISSN 0031-5362

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The revolution in microscopy came in 1930 with the invention of electron microscope. Since then, we can study specimens on ultrastructural and even atomic level. Besides transmission electron microscopy (TEM), there are other types of electron microscopes that are not discussed in this review. Here, we have described basic procedures for TEM sample preparation, which include tissue sample preparation, chemical fixation of tissue with fixatives, cryo-fixation performed by quick freezing, dehydration with ethanol, infiltration with transitional solvents, resin embedding and polymerization, processing of polymerized specimens, sectioning of samples with ultramicrotome, positive and negative contrasting of samples, immunolabeling, and imaging. Such collection of methods can be useful for novices in transmission electron microscopy.

Item Type: Article
Uncontrolled Keywords: TEM; sample preparation; fixation; resin embedding; contrasting; ultramicrotome
Subjects: NATURAL SCIENCES > Biology
INTERDISCIPLINARY AREAS OF KNOWLEDGE > Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area
Divisions: Division of Molecular Biology
Project titleProject leaderProject codeProject type
Procjena fotosintetske aktivnosti i fotozaštitnog potencijala TROL-FNR razdjelnice-FUTURE PLANTHrvoje FulgosiIP-2020-02-8590HRZZ
Depositing User: Lea Vojta
Date Deposited: 07 Feb 2024 10:08
DOI: 10.18054/pb.v125i1-2.25293

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