Dumančić, Ena; Vojta, Lea; Fulgosi, Hrvoje (2023) Beginners guide to sample preparation techniques for transmission electron microscopy. Periodicum Biologorum, 125 (1-2). pp. 123-131. ISSN 0031-5362
![[img]](http://fulir.irb.hr/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
- article
Available under License Creative Commons Attribution. Download (207kB) |
Abstract
The revolution in microscopy came in 1930 with the invention of electron microscope. Since then, we can study specimens on ultrastructural and even atomic level. Besides transmission electron microscopy (TEM), there are other types of electron microscopes that are not discussed in this review. Here, we have described basic procedures for TEM sample preparation, which include tissue sample preparation, chemical fixation of tissue with fixatives, cryo-fixation performed by quick freezing, dehydration with ethanol, infiltration with transitional solvents, resin embedding and polymerization, processing of polymerized specimens, sectioning of samples with ultramicrotome, positive and negative contrasting of samples, immunolabeling, and imaging. Such collection of methods can be useful for novices in transmission electron microscopy.
| Item Type: | Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Uncontrolled Keywords: | TEM; sample preparation; fixation; resin embedding; contrasting; ultramicrotome | ||||||||
| Subjects: | NATURAL SCIENCES > Biology INTERDISCIPLINARY AREAS OF KNOWLEDGE > Biotechnology in Biomedicine (natural science, biomedicine and healthcare, bioethics area |
||||||||
| Divisions: | Division of Molecular Biology | ||||||||
| Projects: |
|
||||||||
| Depositing User: | Lea Vojta | ||||||||
| Date Deposited: | 07 Feb 2024 10:08 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/8532 | ||||||||
| DOI: | 10.18054/pb.v125i1-2.25293 |
Actions (login required)
 |
View Item |
Altmetric
Altmetric
