Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses

Korolija, Marina; Hadžija, Mirko; Pavković, Pajica; Kapitanović, Sanja; Popović Hadžija, Marijana (2008) Improved Performances of Polyacrylamide Gel by Addition of a New Type of Polymer: Detection of Allelic Losses. Croatica Chemica Acta, 81 (3). pp. 437-441. ISSN 0011-1643

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Abstract

The aim of this study was to improve detection of heterozygous samples and loss of heterozygosity using a new type of gel polymer. A total of 60 samples of normal and tumor colon tissue were tested. Eletrophoresis of amplified loci was performed on a standard acrylamide / N,N-methylenebisacrylamide gel, and the same gel containing Spreadex polymer, NAB (native acrylamide-bis). Standard gel displayed 36 samples of normal tissue as homozygous. Electrophoresis of the same samples on gel with addition of polymer revealed 12/36 heterozygous samples. Five of 12 matched tumor samples displayed loss of heterozygosity that could not be detected on standard gel, because of its lower resolving power. Electrophoresis on a new polymer presents a simple, inexpensive, reproducible and non-radioactive method for loss of heterozygosity detection, with improved performances such as increased sensitivity, decreased amount of a sample and improved separation of closely spaced bands. Application of the polymer may contribute to a vide variety of diagnostic procedures.

Item Type:	Article
Uncontrolled Keywords:	DPC4; loss of heterozygosity; allele; Spreadex polymer NAB; tumor-suppressor gene; colon adenocarcinoma; colorectal-cancer; chromosome 18q; cell carcinoma; heterozygosity; mutations; identification; expression; dpc4/smad4
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	Division of Molecular Medicine
Projects:	Project title Project leader Project code Project type Dobivanje struktura nalik Langerhansovim otočićima iz matičnih stanica miša [15065] Mirko Hadžija 098-0982464-2460 MZOS Molekularna genetika i farmakogenetika gastrointestinalnih tumora [200570] Sanja Kapitanović 098-0982464-2508 MZOS
Depositing User:	Marijana Popović Hadžija
Date Deposited:	23 Oct 2013 08:31
URI:	http://fulir.irb.hr/id/eprint/828

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