Sample preparation and culture conditions effects on MALDI-TOF MS identification of bacteria: A review

Topić Popović, Natalija; Kazazić, Snježana; Bojanić, Krunoslav; Strunjak-Perović, Ivančica; Čož-Rakovac, Rozelindra (2023) Sample preparation and culture conditions effects on MALDI-TOF MS identification of bacteria: A review. Mass spectrometry reviews, 42 (5). pp. 1589-1603. ISSN 0277-7037

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Official URL: https://doi.org/10.1002/mas.21739

Abstract

Matrix-assisted laser desorption/ionization time- of-flight mass spectrometry(MALDI-TOF MS) is an excellent tool for bacterial identification. It allows high throughput, sensitive and specific applications in clinical diagnostics and environ- mental research. Currently, there is no optimal standardized protocol for sample preparation and culture conditions to profile bacteria. The performance of MALDITOF MS is affected by several variables, such as sample preparation, culture media and culture conditions, incubation time/growth stage, incubation temperature, high salt content, blood in the culture media, and others. This review thus aims to clarify why a uniformed protocol is not plausible, to assess the effects these factors have onMALDITOF MS identification score, and discuss possible optimizations for its methodology, in relation to specific bacterial representatives and strain requirements.

Item Type:

Article

Uncontrolled Keywords:

Bacteria ; Culture conditions ; Diagnostics ; MALDI-TOF MS ; Sample preparation

Subjects:

NATURAL SCIENCES > Biology
BIOMEDICINE AND HEALTHCARE > Veterinary Medicine
BIOTECHNICAL SCIENCES > Biotechnology

Divisions:

Division of Materials Chemistry
Division of Materials Physics

Projects: