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Glucose, Insulin and Oxygen Modulate Expression of Serotonin-Regulating Genes in Human First-Trimester Trophoblast Cell Line ACH-3P

Perić, Maja; Horvatiček, Marina; Tandl, Veronika; Bečeheli, Ivona; Majali-Martinez, Alejandro; Desoye, Gernot; Štefulj, Jasminka (2023) Glucose, Insulin and Oxygen Modulate Expression of Serotonin-Regulating Genes in Human First-Trimester Trophoblast Cell Line ACH-3P. Biomedicines, 11 (6). ISSN 2227-9059

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Serotonin signaling plays an important role in regulating development and functions of the placenta. We hypothesized that metabolic disturbances associated with maternal obesity and/or gestational diabetes mellitus (GDM) affect placental serotonin homeostasis. Therefore, we examined the effects of high glucose (25 mM) and insulin (10 nM)—two hallmarks of maternal obesity and GDM—on mRNA expression of key regulators of serotonin homeostasis, including serotonin transporter (SERT), tryptophan hydroxylase 1 (TPH1), and monoamine oxidase A (MAOA), in the first-trimester trophoblast cell line ACH-3P, focusing on oxygen levels characteristic of early human placental development. Glucose downregulated expression of SERT and MAOA independently of oxygen level and upregulated expression of TPH1 at 6.5% oxygen but not at 2.5% oxygen. Compared to 6.5% oxygen, 2.5% oxygen upregulated SERT and downregulated TPH1 expression, with no effect on MAOA expression. Insulin upregulated SERT only at 2.5% oxygen but had no effect on TPH1 and MAOA expression. These results suggest that maternal metabolic alterations in early pregnancy may be a driving force for changes in placental serotonin homeostasis.

Item Type: Article
Uncontrolled Keywords: gestational diabetes mellitus ; obesity ; 5-HT ; 5-HTT ; placenta ; human trophoblasts ; early pregnancy ; hyperglycemia ; hyperinsulinemia ; hypoxia
Subjects: NATURAL SCIENCES > Biology
Divisions: Division of Molecular Biology
Project titleProject leaderProject codeProject type
Utjecaj majčinog metaboličkog stanja na serotoninski sustav posteljice i neonatusa: od metilacije DNA do funkcije proteinaŠtefulj, JasminkaIP-2018-01-6547HRZZ
Depositing User: Jasminka Štefulj
Date Deposited: 06 Jul 2023 12:25
DOI: 10.3390/biomedicines11061619

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