Rhamnus intermedia Steud. et Hochst. - a New Source of Bioactive Phytochemicals

Šamec, Dunja; Kremer, Dario; Grúz, Jiří; Jurišić Grubešić, Renata; Piljac Žegarac, Jasenka (2012) Rhamnus intermedia Steud. et Hochst. - a New Source of Bioactive Phytochemicals. Croatica Chemica Acta, 85 (2). pp. 125-129. ISSN 0011-1643

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Abstract

Antioxidant capacity and various classes of phenolic antioxidants were quantified in leaf and bark methanol extracts of a medicinal plant, Rhamnus intermedia Steud. et Hochst., whose phytochemical composition is unknown. Three well-established assays were used to determine the antioxidant potency of the extracts: the Ferric Reducing/Antioxidant Power assay (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) assay, as well as oxygen radical absorbance capacity (ORAC) assay. Total phenol (TP, Folin-Ciocalteu assay), total flavonoid (IF, colorimetric assay with AlCl3) and free phenolic acid (UPLC-MS/MS) content of extracts was quantified. In comparison to bark extracts, leaf extracts exhibited significantly higher TP and IF contents, as well as higher antioxidant capacity according to all four assays. Ten free phenolic acids were quantified in leaf extracts while six were detected in bark extracts. The most abundant phenolic acid in leaves was vanilic acid (1647.06 +/- 79.35 mu mol g(-1) DW; DW, dry weight) while salycilic acid was the most concentrated in the bark (111.10 +/- 14.14 mu mol g(-1) DW).

Item Type:	Article
Uncontrolled Keywords:	Rhamnus intermedia; antioxidant activity; total phenols; total flavonoids; phenolic acids; antioxidant capacity; medicinal-plants; polyphenol; prevention; assays; foods
Subjects:	NATURAL SCIENCES > Chemistry
Divisions:	Division of Molecular Biology
Projects:	Project title Project leader Project code Project type Mikromorfološka i kemotaksonomska istraživanja nekih vrsta porodice Lamiaceae [240541] Dario Kremer 006-0000000-3178 MZOS Molekularna regulacija biljnog razvitka [200002] Branka Salopek-Sondi 098-0982913-2829 MZOS
Depositing User:	Jasenka Piljac Žegarac
Date Deposited:	14 Oct 2013 08:38
URI:	http://fulir.irb.hr/id/eprint/779
DOI:	10.5562/cca1946

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