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Characterization of Third Generation Cephalosporin- and Carbapenem-Resistant Aeromonas Isolates from Municipal and HospitalWastewater

Drk, Sara; Puljko, Ana; Dželalija, Mia; Udiković-Kolić, Nikolina (2023) Characterization of Third Generation Cephalosporin- and Carbapenem-Resistant Aeromonas Isolates from Municipal and HospitalWastewater. Antibiotics, 12 (3). ISSN 2079-6382

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Antibiotic resistance (AR) remains one of the greatest threats to global health, and Aeromonas species have the potential to spread AR in the aquatic environment. The spread of resistance to antibiotics important to human health, such as third-generation cephalosporins (3GCs) and carbapenems, is of great concern. We isolated and identified 15 cefotaxime (3GC)- and 51 carbapenemresistant Aeromonas spp. from untreated hospital and treated municipal wastewater in January 2020. The most common species were Aeromonas caviae (58%), A. hydrophila (17%), A. media (11%), and A. veronii (11%). Almost all isolates exhibited a multidrug-resistant phenotype and harboured a diverse plasmidome, with the plasmid replicons ColE, IncU, and IncR being the most frequently detected. The most prevalent carbapenemase gene was the plasmid-associated blaKPC-2 and, for the first time, the blaVIM-2, blaOXA-48, and blaIMP-13 genes were identified in Aeromonas spp. Among the 3GC-resistant isolates, the blaGES-5 and blaMOX genes were the most prevalent. Of the 10 isolates examined, three were capable of transferring carbapenem resistance to susceptible recipient E. coli. Our results suggest that conventionally treated municipal and untreated hospital wastewater is a reservoir for 3GC- and carbapenem-resistant, potentially harmful Aeromonas spp. that can be introduced into aquatic systems and pose a threat to both the environment and public health.

Item Type: Article
Uncontrolled Keywords: Aeromonas; hospital wastewater; municipal wastewater; ESBL; AmpC; carbapenemase; plasmid transfer
Subjects: BIOTECHNICAL SCIENCES > Biotechnology
BIOTECHNICAL SCIENCES > Biotechnology > Molecular Biotechnology
BIOTECHNICAL SCIENCES > Interdisciplinary Technical Sciences
Divisions: Division for Marine and Enviromental Research
Project titleProject leaderProject codeProject type
Antibiotička rezistencija u uređajima za obradu otpadnih voda u Hrvatskoj: naglasak na ß-laktamaze proširenog spektra i karbapenemaze-WasteCareNikolina Udiković Kolić; Stela Križanović; Ana Maravić; Marko Jelić; Josipa Kosić-Vukšić; Tamara Jurina; Marko Virta; Gisle VestergaardIP-2019-04-5539HRZZ
Depositing User: Ana Puljko
Date Deposited: 03 Apr 2023 13:54
DOI: antibiotics12030513

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