Characterisation and toxicological activity of three different Pseudo-nitzschia species from the northern Adriatic Sea (Croatia)

Smodlaka Tanković, Mirta; Baričević, Ana; Gerić, Marko; Domijan, Ana-Marija; Marić Pfannkuchen, Daniela; Kužat, Nataša; Ujević, Ivana; Kuralić, Malissa; Rožman, Marko; Matković, Katarina; Novak, Matjaž; Zegura, Bojana; Pfannkuchen, Martin; Gajski, Goran (2022) Characterisation and toxicological activity of three different Pseudo-nitzschia species from the northern Adriatic Sea (Croatia). Environmental research, 214 . ISSN 0013-9351

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Abstract

Diatoms of the genus Pseudo-nitzschia are cosmopolitans spread in seas and oceans worldwide, with more than 50 described species, dozens of which have been confirmed to produce domoic acid (DA). Here, we characterized and investigated the toxicological activity of secondary metabolites excreted into the growth media of different Pseudo-nitzschia species sampled at various locations in the northern Adriatic Sea (Croatia) using human blood cells under in vitro conditions. The results revealed that three investigated species of the genus Pseudo-nitzschia were capable of producing DA indicating their toxic potential. Moreover, toxicological data suggested all three Pseudo-nitzschia species can excrete toxic secondary metabolites into the surrounding media in addition to the intracellular pools of DA, raising concerns regarding their toxicity and environmental impact. In addition, all three Pseudo-nitzchia species triggered oxidative stress, one of the mechanisms of action likely responsible for the DNA damage observed in human blood cells. In line with the above stated, our results are of great interest to environmental toxicologists, the public and policy makers, especially in light of today's climate change, which favours harmful algal blooms and the growth of DA producers with a presumed negative impact on the public health of coastal residents.

Item Type:	Article
Uncontrolled Keywords:	Pseudo-nitzschia ; Domoic acid ; human peripheral blood cells ; Cyto/genotoxicity ; environmental safety
Subjects:	NATURAL SCIENCES > Interdisciplinary Natural Sciences > Environmental Science
Divisions:	Division of Physical Chemistry
Projects:	Project title Project leader Project code Project type Životne strategije fitoplanktona u sjevernom Jadranu-P-limit Martin Pfannkuchen UIP-2014-09-6563 HRZZ Molekularne životne strategije fitoplanktona u Jadranu-Adri LIFE Daniela Marić Pfannkuchen UIP-2020-02-7868 HRZZ
Depositing User:	Marko Rožman
Date Deposited:	05 Dec 2022 15:11
URI:	http://fulir.irb.hr/id/eprint/7632
DOI:	10.1016/j.envres.2022.114108

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