Novosel, Nives; Mišić Radić, Tea; Levak Zorinc, Maja; Zemla, Joanna; Lekka, Malgorzata; Vrana Špoljarić, Ivna; Gašparović, Blaženka; Horvat, Lucija; Kasum, Damir; Legović, Tarzan; Žutinić, Petar; Gligora Udovič, Marija; Ivošević DeNardis, Nadica (2022) Salinity-induced chemical, mechanical, and behavioral changes in marine microalgae. Journal of applied phycology, 34 . pp. 1293-1309. ISSN 0921-8971
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Abstract
This study examines how salinity reduction triggers the response of three marine microalgae at the molecular and unicellular levels in terms of chemical, mechanical, and behavioral changes. At the lowest salinity, all microalgal species exhibited an increase in membrane sterols and behaved stifer. The glycocalyx-coated species Dunaliella tertiolecta was surrounded by a thick actin layer and showed the highest physiological activity, negatively afecting cell motility and indicating the formation of the palmella stage. The lipid content of membrane and the hydrophobicity of cell were largely preserved over a wide range of salinity, confrming the euryhaline nature of Dunaliella. The species with calcite- encrusted theca Tetraselmis suecica exhibited the highest hydrophobicity at the lowest salinity of all cells examined. At salinity of 19, the cells of T. suecica showed the lowest growth, fagellar detachment and the lowest cell speed, the highest physiological activity associated with a dense network of extracellular polymeric substances, and a decrease in membrane lipids, which could indicate develepment of cyst stage. The organosilicate encrusted species Cylindrotheca closterium appeared to be salinity tolerant. It behaved hydrophobically at lower salinity, whereas becoming hydrophilic at higher salinity, which might be related to a molecular change in the released biopolymers. This study highlighted the interplay between chemistry and mechanics that determines functional cell behavior and shows that cell surface properties and behavior could serve as stress markers for marine biota under climate change.
| Item Type: | Article | ||||||||
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| Uncontrolled Keywords: | Extracellular polymeric substances ; Hyposalinity ; Lipids ; Microalgae ; Motility ; Nanomechanics | ||||||||
| Subjects: | NATURAL SCIENCES > Chemistry NATURAL SCIENCES > Biology NATURAL SCIENCES > Interdisciplinary Natural Sciences BIOTECHNICAL SCIENCES > Biotechnology |
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| Divisions: | Division for Marine and Enviromental Research Division of Molecular Biology |
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| Depositing User: | Tea Mišić Radić | ||||||||
| Date Deposited: | 14 Jul 2022 12:25 | ||||||||
| URI: | http://fulir.irb.hr/id/eprint/7478 | ||||||||
| DOI: | 10.1007/s10811-022-02734-x |
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